Alexa Fluor 532 excitation shown as dotted line and emission shown as solid green-yellow histogram
3532555/20534553(in buffer): 2
(in antifade): 3
microscopy, flow cytometry, fluorescence microplate reader

Alexa Fluor 532 is a yellow-fluorescent dye with excitation designed for use with the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

Alexa Fluor 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.

We offer Alexa Fluor 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.

Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)   Search Alexa Fluor 532 primary antibodies

labeled human mononuclear cells

Figure 1. Human mononuclear cells were stained with mouse anti human CD4 biotin, washed, stained with streptavidin, Alexa Fluor 532 conjugate, washed, and analyzed by flow cytometry using 532 nm excitation.

cells labeled with anti-tubulin shown with yellow fluorescence

Figure 2. A bovine pulmonary artery endothelial cell labeled with mouse monoclonal anti-a-tubulin antibody in combination with Alexa Fluor 532 goat anti-mouse IgG antibody to stain microtubules. The image was acquired using a bandpass filter set (excitation/emission 535 +- 17.5/590 +- 17.5 nm).

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For Research Use Only. Not for use in diagnostic procedures.