Alexa Fluor® 532 dyeAlexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

Alexa Fluor 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.


We offer Alexa Fluor 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.

Alexa Fluor 532 dye dashboard

Initial brightness

Alexa Fluor 532 dye is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM.



Photostability in buffer


Photostability in antifade


Performance in SRM



Human mononuclear cells were stained with mouse anti human CD4 biotin, washed, stained with streptavidin, Alexa Fluor 532 conjugate, washed, and analyzed by flow cytometry using 532 nm excitation.

Alexa Fluor 532 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Alexa Fluor 532 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target proteinKitCat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1Zenon Alexa Fluor 532 Mouse IgG1 Labeling KitZ25003
<1–20 µg rabbit IgGZenon Alexa Fluor 532 Rabbit IgG Labeling KitZ25303
Conventional antibody/protein labeling
100 µg antibody/proteinAlexa Fluor 532 Antibody/Protein Labeling KitA20182
1 mg antibody/proteinAlexa Fluor 532 Antibody/Protein Labeling KitA10236
Alexa Fluor Dye stained cell

Alexa Fluor dye family

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.