Alexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.
Alexa Fluor 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.
We offer Alexa Fluor 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
Alexa Fluor 532 dye dashboard
|Alexa Fluor 532 dye is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM.
Photostability in buffer
Photostability in antifade
Performance in SRM
Alexa Fluor 532 dye products
We offer a broad range of Alexa Fluor 532 products including protein and antibody labeling kits, secondary antibodies, and bioconjugates:
- Super-resolution microscopy (SRM) applications
- Antibody and protein labeling kits
- Streptavidin conjugate for signal amplification
- Phalloidin conjugate for cytoskeleton staining
Alexa Fluor 532 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor 532 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon Alexa Fluor 532 Mouse IgG1 Labeling Kit||Z25003|
|<1–20 µg rabbit IgG||Zenon Alexa Fluor 532 Rabbit IgG Labeling Kit||Z25303|
|Conventional antibody/protein labeling|
|100 µg antibody/protein||Alexa Fluor 532 Antibody/Protein Labeling Kit||A20182|
|1 mg antibody/protein||Alexa Fluor 532 Antibody/Protein Labeling Kit||A10236|
5 steps resources
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.