Alexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.
Alexa Fluor 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.
We offer Alexa Fluor 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
Alexa Fluor 532 dye dashboard
|Alexa Fluor 532 dye is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM.
Photostability in buffer
Photostability in antifade
Performance in SRM
Alexa Fluor 532 dye products
We offer a broad range of Alexa Fluor 532 products including labeling kits, secondary antibodies, and bioconjugates:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits
- Protein labeling kits
- Streptavidin conjugate for signal amplification
- Phalloidin conjugate for cytoskeleton staining
Alexa Fluor 532 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor 532 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon Alexa Fluor 532 Mouse IgG1 Labeling Kit||Z25003|
|<1–20 µg rabbit IgG||Zenon Alexa Fluor 532 Rabbit IgG Labeling Kit||Z25303|
|Conventional antibody/protein labeling|
|100 µg antibody/protein||Alexa Fluor 532 Antibody/Protein Labeling Kit||A20182|
|1 mg antibody/protein||Alexa Fluor 532 Antibody/Protein Labeling Kit||A10236|
Alexa Fluor dye family
Illuminating research for over 15 years
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Molecular Probes Handbook
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.