A= 10 µg/ml Primary Antibody, No BRDU incubation 2nd Antibody: A11002 1:200 dilution (DAPI=Blue), B= No Primary antibody, Hela cells incubated in 30 µg/ml for 24hrs, 2nd Antibody: A11002 1:200 dilution (DAPI= Blue), C= 2.5 µg/ml Primary Antibody , Hela cells incubated in 30 µg/ml for 24hrs2nd Antibody: A11002 (Only Blue/Dapi shown), D= 2.5 µg/ml Primary Antibody , Hela cells incubated in 30 µg/ml for 24hrs 2nd Antibody: A11002 (Only red/Alexa 532 shown), D= 2.5 µg/ml Primary Antibody , Hela cells incubated in 30 µg/ml for 24hrs 2nd Antibody: A11002 (Only red/Alexa 532 shown).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 532|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 7 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging.||Szymborska A,de Marco A,Daigle N,Cordes VC,Briggs JA,Ellenberg J||Science (New York, N.Y.) (341:655)||2013|
|Not Applicable||Not Cited||Investigating cellular structures at the nanoscale with organic fluorophores.||van de Linde S,Aufmkolk S,Franke C,Holm T,Klein T,Löschberger A,Proppert S,Wolter S,Sauer M||Chemistry and biology (20:8)||2013|
|Not Applicable||Not Cited||Single molecule imaging of protein molecules in nanopores.||Ma C,Yeung ES||Analytical chemistry (82:478)||2010|
|Not Applicable||Not Cited||Super-resolution imaging with small organic fluorophores.||Heilemann M,van de Linde S,Mukherjee A,Sauer M||Angewandte Chemie (International ed. in English) (48:6903)||2009|
|Not Applicable||Not Cited||A microengraving method for rapid selection of single cells producing antigen-specific antibodies.||Love JC,Ronan JL,Grotenbreg GM,van der Veen AG,Ploegh HL||Nature biotechnology (24:703)||2006|
|Not Applicable||Not Cited||Direct production and purification of T7 phage display cloned proteins selected and analyzed on microarrays.||Nowak JE,Chatterjee M,Mohapatra S,Dryden SC,Tainsky MA||BioTechniques (40:220)||2006|
|Not Applicable||Not Cited||N-cadherin-dependent cell-cell contact regulates Rho GTPases and beta-catenin localization in mouse C2C12 myoblasts.||Charrasse S,Meriane M,Comunale F,Blangy A,Gauthier-Rouvière C||The Journal of cell biology (158:953)||2002|