T cell function is dependent upon and controlled by a variety of inducible structures that may have stimulatory (ICOS, OX40, and 4-1BB) or inhibitory (cytotoxic T-lymphocyte antigen [CTLA-4/CD152], B and T lymphocyte attenuator [BTLA/CD272]) potential. Some of these receptors act as either immune inhibitors or activators depending upon the stimulatory environment.

Most co-stimulatory and inhibitory molecules are organized into two families based upon their sequence similarities. The Ig superfamily includes the co-stimulatory receptor, CD28 and inducible costimulator (ICOS/CD278), and the inhibitory receptors, (CD152), programmed cell death-1 (PD-1/CD279), CD272 and CD160. The tumor necrosis factor (TNF) superfamily includes the co-stimulatory receptors CD40, CD134 (OX40), and herpes virus entry mediator (CD270 HVEM).

Costimulatory and Inhibitory Interactions Control T Cell Response

Co-stimulation - Receptor - Ligand Quick Guide

ReceptorHuman FG Ab
Cat. No.
Mouse FG Ab
Cat. No.
FamilyExpressionSignalLigandHuman FG Ab
Cat. No.
Mouse FG Ab
Cat. No.
Ligand Expression
CD216-002916-0021IgSFT, B and NK cells+CD58 (LFA-3)- -- -Hematopoietic,
non-hematopoietic cells
CD416-004916-0041IgSFT subset and Macro/ Mono+ and -MHC class II (I-A/I-E)- -16-5321B, Marco/Mono, DC and human T cells
CD2816-028916-0281IgSFT cells+CD80 (B7-1)
CD86 (B7-2)
16-0809
16-0869
16-0801
16-0861
16-0862
B, Macro/Mono, DC and activated T cells
CD152 (CTLA-4)16-152916-1521IgSFB and activated T cells-CD80 (B7-1) CD86 (B7-2)16-0809
16-0869
16-0801
16-0861
16-0862
B, Marco/Mono, DC and activated T cells
CD1601609
(clone BY55)
16-1601IgSFT, NK and NKT cells-CD270 (HVEM)
MHC class I (H-2Db)
5969 (clone HVEM-122)
9958 (clone A4)
5962 (clone
LH1) 16-5957
16-5999
T, B, DC, Macro/Mono and NK cells
CD223 (Lag-3) 16-2231IgSFActivated T, B and NK cells-MHC class II (I-A/I-E)- -16-5321B, Marco/Mono, DC and human T cells
CD244 (2B4)16-5838
16-2449
244F4IgSFNK, T subset, Basophils and Macro/Mono+ and -CD4816-048916-0481Most leukocytes
CD272 (BTLA)16-597916-5950IgSFActivated T, Th1, B and DC cells-CD270 (HVEM)5969 (clone HVEM-122)5962
(clone LH1)
T, B, DC, Macro/Mono and NK cells
CD274
(B7-H1/PD-L1)
- -- -IgSFB, Marco/Mono, DC, T and NK cells-CD80 (B7-1)16-080916-0801B, Macro/Mono, DC and activated T cells
CD278 (ICOS)16-994816-9942
16-9949
IgSFActivated T, Th2 and NKT cells+CD275
(B7-H2, ICOS-L)
16-588916-5985B, Marco/Mono, DC and endothelial cells
CD279 (PD-1)16-998916-5982
16-9985
IgSFActivated T, activated B, DC and Macro/Mono-CD274 (B7-H1, PD-L1) CD273 (B7-DC, PD-L2)16-5983
16-5888
16-9982
16-5986”
B, Marco/Mono, DC, T and NK, Marco/Mono and DC cells
TIM33109 (clone F38-2E2)16-5871IgSFT, B, DC, Macro/Mono and NK cells-Galectin 9- -- -T, Treg and others
CD2716-0271
16-0272
16-0271
16-0272
TNFRSFT, B subset, NK subset and HSC+CD70- -16-0701Activated T, B and DC cells
CD300309 (clone Ber-H2)16-0301TNFRSFActivated T, B and NK cells+CD153 (CD30L)- -16-1531B and activated T cells
CD134 (OX40)1347 (clone ACT-35)16-1341TNFRSFActivated T, B, DC and Marco/Monos+ (- for Treg)CD252 (OX40
Ligand)
- -16-5905Activated T, Treg, NKT, NK and neutrophils
CD137 (4-1BB)1379 (clone 4B4)16-1371TNFRSFActivated T, Macro/ Mono, DC and NK cells+CD137 Ligand (4-1BBL)- -16-5901B, DC and Macro/Mono
CD154 (CD40L)16-154816-1541TNFRSFActivated T, B and platelets cells+CD4016-040916-0402B, DC, Macro/Mono and endothelial cells
CD270 (HVEM)5969
(clone HVEM-122)
5962
(clone LH1)
TNFRSFT, B, DC, Macro/Mono, and NK cells+CD258 (LIGHT) CD272 (BTLA)
CD160
2589
(clone 7-3)
16-5979
1609
(clone BY55)
- -
16-5950
16-1601
Activated T and B, DC, Marco/Monos and
NK cells
CD357 (GITR)5875 (clone AITR)16-5874TNFRSFActivated T, Treg and NK cells+GITR Ligand- -5854 (clone
YGL 386)
B, DC, Macro/Mono and endothelial cells
Lymphotoxon β
Receptor
- -16-5671TNFRSFDC, Macro/Mono and stromal cells+CD258 (LIGHT)
LTαβ
2589
(clone 7-3) - -
- -
- -
Activated T and B, DC, Marco/Monos and
NK cells