Invitrogen™

Zenon™ Mouse IgG1 Labeling Kit

Catalog number: Z25002
Invitrogen™

Zenon™ Mouse IgG1 Labeling Kit

Catalog number: Z25002
Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.
 
Catalog Number
Z25002
also known as Z-25002
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
496/519
Label or Dye
Alexa Fluor 488
Price (USD)
477.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission496/519
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 488
Label TypeAlexa Fluor
ColorGreen
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25000
also known as Z-25000
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
346/442
Label or Dye
Alexa Fluor 350
Price (USD)
457.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission346/442
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 350
Label TypeAlexa Fluor Dyes
ColorBlue
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25004
also known as Z-25004
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
556/573
Label or Dye
Alexa Fluor 546
Price (USD)
462.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission556/573
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 546
Label TypeAlexa Fluor
ColorOrange
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25005
also known as Z-25005
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
555/565
Label or Dye
Alexa Fluor 555
Price (USD)
470.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission555/565
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 555
Label TypeAlexa Fluor
ColorOrange
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25006
also known as Z-25006
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
578/603
Label or Dye
Alexa Fluor 568
Price (USD)
471.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission578/603
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 568
Label TypeAlexa Fluor
ColorRed-Orange
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25007
also known as Z-25007
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
590/617
Label or Dye
Alexa Fluor 594
Price (USD)
471.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission590/617
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 594
Label TypeAlexa Fluor
ColorRed
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25008
also known as Z-25008
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
650/668
Label or Dye
Alexa Fluor 647
Price (USD)
481.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission650/668
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 647
Label TypeAlexa Fluor
ColorFar-Red
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25011
also known as Z-25011
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
696/719
Label or Dye
Alexa Fluor 700
Price (USD)
407.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission696/719
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 700
Label TypeAlexa Fluor
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25013
also known as Z-25013
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
402/421
Label or Dye
Alexa Fluor 405
Price (USD)
407.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity50 Reactions
Excitation/Emission402/421
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 405
Label TypeAlexa Fluor
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Catalog Number
Z25021
also known as Z-25021
Unit Size
1 kit
Quantity
10 Reactions
Excitation/Emission
496/668
Label or Dye
Alexa Fluor 647-R-Phycoerythrin
Price (USD)
407.00
Your Price:
Availability
***
Full specifications
Quantity
Quantity10 Reactions
Excitation/Emission496/668
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 647-R-Phycoerythrin
Label TypeAlexa Fluor-R-Phycoerythrin
ColorFar-Red
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Showing 10 of 16Show more
Catalog NumberSpecificationsUnit SizeQuantityExcitation/EmissionLabel or DyePrice (USD)AvailabilityQuantity
Z25002
also known as Z-25002
Full specifications
1 kit50 Reactions496/519Alexa Fluor 488
477.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission496/519
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 488
Label TypeAlexa Fluor
ColorGreen
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25000
also known as Z-25000
Full specifications
1 kit50 Reactions346/442Alexa Fluor 350
457.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission346/442
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 350
Label TypeAlexa Fluor Dyes
ColorBlue
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25004
also known as Z-25004
Full specifications
1 kit50 Reactions556/573Alexa Fluor 546
462.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission556/573
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 546
Label TypeAlexa Fluor
ColorOrange
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25005
also known as Z-25005
Full specifications
1 kit50 Reactions555/565Alexa Fluor 555
470.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission555/565
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 555
Label TypeAlexa Fluor
ColorOrange
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25006
also known as Z-25006
Full specifications
1 kit50 Reactions578/603Alexa Fluor 568
471.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission578/603
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 568
Label TypeAlexa Fluor
ColorRed-Orange
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25007
also known as Z-25007
Full specifications
1 kit50 Reactions590/617Alexa Fluor 594
471.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission590/617
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 594
Label TypeAlexa Fluor
ColorRed
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25008
also known as Z-25008
Full specifications
1 kit50 Reactions650/668Alexa Fluor 647
481.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission650/668
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 647
Label TypeAlexa Fluor
ColorFar-Red
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25011
also known as Z-25011
Full specifications
1 kit50 Reactions696/719Alexa Fluor 700
407.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission696/719
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 700
Label TypeAlexa Fluor
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25013
also known as Z-25013
Full specifications
1 kit50 Reactions402/421Alexa Fluor 405
407.00
Your Price:
***
Quantity50 Reactions
Excitation/Emission402/421
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 405
Label TypeAlexa Fluor
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Z25021
also known as Z-25021
Full specifications
1 kit10 Reactions496/668Alexa Fluor 647-R-Phycoerythrin
407.00
Your Price:
***
Quantity10 Reactions
Excitation/Emission496/668
Labeling Scale< 1–20 μg
Product LineZenon
Label or DyeAlexa Fluor 647-R-Phycoerythrin
Label TypeAlexa Fluor-R-Phycoerythrin
ColorFar-Red
Labeling TargetIgG1
SpeciesMouse
Unit Size1 kit
Showing 10 of 16Show more
Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG1 labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG1 primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.
Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:

• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 µg primary antibody
• Simple—no purification required
• Flexible — choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 µg in 2 µL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.
Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.
Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.
Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.
Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.
Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in refrigerator (2–8°C) and protect from light

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