Informational:Informational:New Zenon Alexa Fluor Plus Labeling Reagents are superior in three ways: improved bioconjugation for brighter signal, redesigned protein for robust screening regardless of IgG isotype, and new, more sensitive fluorophores.
Invitrogen™

Zenon™ Mouse IgG1 Labeling Kits

Catalog number: Z25002
Have Questions?
Invitrogen™

Zenon™ Mouse IgG1 Labeling Kits

Catalog number: Z25002
Have Questions?
Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.
 
Catalog Number
Z25002
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
496/519 nm
Label or Dye
Alexa Fluor 488
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission496/519 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
555/565 nm
Label or Dye
Alexa Fluor 555
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission555/565 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 555
ColorOrange
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Z25006
also known as Z-25006
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
578/603 nm
Label or Dye
Alexa Fluor 568
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission578/603 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 568
ColorRed-Orange
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
590/617 nm
Label or Dye
Alexa Fluor 594
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission590/617 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 594
ColorRed
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
650/668 nm
Label or Dye
Alexa Fluor 647
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission650/668 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 647
ColorFar-Red
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
696/719 nm
Label or Dye
Alexa Fluor 700
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission696/719 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 700
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
402/421 nm
Label or Dye
Alexa Fluor 405
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission402/421 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 405
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Catalog Number
Z25051
also known as Z-25051
Unit Size
1 kit
Quantity
25 Reactions
Excitation/Emission
650/660 nm
Label or Dye
APC (Allophycocyanin)
Price (USD)
Availability
-
Full specifications
Quantity
Quantity25 Reactions
Excitation/Emission650/660 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAPC (Allophycocyanin)
ColorRed
Labeling TargetIgG1
SpeciesMouse
Label TypePE & APC
Unit Size1 kit
Catalog Number
Z25052
also known as Z-25052
Unit Size
1 kit
Quantity
50 Reactions
Excitation/Emission
Label or Dye
Biotin
Price (USD)
Availability
-
Full specifications
Quantity
Product TypeLabeling Kit
Quantity50 Reactions
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeBiotin
ColorColorless
Labeling TargetIgG1
SpeciesMouse
Label TypeBiotin & Other Haptens
Unit Size1 kit
Catalog Number
Z25055
also known as Z-25055
Unit Size
1 kit
Quantity
25 Reactions
Excitation/Emission
496, 546, 565/578 nm
Label or Dye
R-PE (R-Phycoerythrin)
Price (USD)
Availability
-
Full specifications
Quantity
Quantity25 Reactions
Excitation/Emission496, 546, 565/578 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeR-PE (R-Phycoerythrin)
ColorRed-Orange
Labeling TargetIgG1
SpeciesMouse
Label TypePE & APC
Unit Size1 kit
Showing 10 of 10
Catalog NumberSpecificationsUnit SizeQuantityExcitation/EmissionLabel or DyePrice (USD)AvailabilityQuantity
Z25002Full specifications
1 kit50 Reactions496/519 nmAlexa Fluor 488
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission496/519 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25005Full specifications
1 kit50 Reactions555/565 nmAlexa Fluor 555
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission555/565 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 555
ColorOrange
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25006
also known as Z-25006
Full specifications
1 kit50 Reactions578/603 nmAlexa Fluor 568
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission578/603 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 568
ColorRed-Orange
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25007Full specifications
1 kit50 Reactions590/617 nmAlexa Fluor 594
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission590/617 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 594
ColorRed
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25008Full specifications
1 kit50 Reactions650/668 nmAlexa Fluor 647
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission650/668 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 647
ColorFar-Red
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25011Full specifications
1 kit50 Reactions696/719 nmAlexa Fluor 700
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission696/719 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 700
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25013Full specifications
1 kit50 Reactions402/421 nmAlexa Fluor 405
-
Product TypeLabeling Kit
Quantity50 Reactions
Excitation/Emission402/421 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAlexa Fluor 405
ColorViolet
Labeling TargetIgG1
SpeciesMouse
Label TypeAlexa Fluor
Unit Size1 kit
Z25051
also known as Z-25051
Full specifications
1 kit25 Reactions650/660 nmAPC (Allophycocyanin)
-
Quantity25 Reactions
Excitation/Emission650/660 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeAPC (Allophycocyanin)
ColorRed
Labeling TargetIgG1
SpeciesMouse
Label TypePE & APC
Unit Size1 kit
Z25052
also known as Z-25052
Full specifications
1 kit50 ReactionsBiotin
-
Product TypeLabeling Kit
Quantity50 Reactions
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeBiotin
ColorColorless
Labeling TargetIgG1
SpeciesMouse
Label TypeBiotin & Other Haptens
Unit Size1 kit
Z25055
also known as Z-25055
Full specifications
1 kit25 Reactions496, 546, 565/578 nmR-PE (R-Phycoerythrin)
-
Quantity25 Reactions
Excitation/Emission496, 546, 565/578 nm
Labeling Scale< 1–20 μg
Product LineZenon™
Label or DyeR-PE (R-Phycoerythrin)
ColorRed-Orange
Labeling TargetIgG1
SpeciesMouse
Label TypePE & APC
Unit Size1 kit
Showing 10 of 10

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG1 labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG1 primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.

Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:
• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 μg primary antibody
• Simple—no purification required
• Flexible—choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 μg in 2 μL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.

Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.

Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.

Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.

Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.

Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Contains 1 vial of Zenon Alexa Fluor 488 mouse IgG1 labeling reagent (250 μL), and 1 vial of Zenon blocking reagent (mouse IgG, 250 μL).

Store in refrigerator (2–8°C) and protect from light.

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