Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence analysis of Beta-Catenin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Beta-Catenin Mouse Monoclonal Antibody (138400) at 1.5µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization of Beta-Catenin and panel e is a no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Mouse , Human , Chicken|
|Published species reactivity||Rat , Mouse , Human , Chicken|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken beta-Catenin.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Western Blot (WB)||See 6 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical demonstration of alteration of β-catenin during tumor metastasis by different mechanisms according to histology in lung cancer.
13-8400 was used in immunohistochemistry - paraffin section to study the regulatory mechanism of beta-catenin on tumor metastasis
|Xu X,Kim JE,Sun PL,Yoo SB,Kim H,Jin Y,Chung JH||Experimental and therapeutic medicine (9:311)||2015|
Aberration of the Wnt signaling pathway in pulmonary fatal adenocarcinoma: a case report.
13-8400 was used in immunohistochemistry - paraffin section to characterize a well-differentiated fetal lung adenocarcinoma from a human
|Li J,Bai X,Zou Y,Hao Y,Xu X||Chinese journal of cancer research = Chung-kuo yen cheng yen chiu (26:E13)||2014|
The role of beta-catenin, TGF beta 3, NGF2, FGF2, IGFR2, and BMP4 in the pathogenesis of mesenteric sclerosis and angiopathy in midgut carcinoids.
13-8400 was used in immunohistochemistry - paraffin section to study the role of several fibrosing-related growth factor in the pathogenesis of mesenteric sclerosis and angiopathy in midgut carcinoids.
|Zhang PJ,Furth EE,Cai X,Goldblum JR,Pasha TL,Min KW||Human pathology (35:670)||2004|
EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study.
13-8400 was used in immunohistochemistry - frozen section and western blot to study the role of EB1 in tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier.
|Tang EI,Mok KW,Lee WM,Cheng CY||Endocrinology (156:680)||2015|
Wnt5A regulates ABCB1 expression in multidrug-resistant cancer cells through activation of the non-canonical PKA/β-catenin pathway.
13-8400 was used in western blot to study the role of Wnt5A signaling in cancer drug resistance
|Hung TH,Hsu SC,Cheng CY,Choo KB,Tseng CP,Chen TC,Lan YW,Huang TT,Lai HC,Chen CM,Chong KY||Oncotarget (5:12273)||2014|
Development of a novel neuroprotective strategy: combined treatment with hypothermia and valproic acid improves survival in hypoxic hippocampal cells.
13-8400 was used in western blot to evaluate a novel neuroprotective strategy in hypoxic hippocampal cells
|Jin G,Liu B,You Z,Bambakidis T,Dekker SE,Maxwell J,Halaweish I,Linzel D,Alam HB||Surgery (156:221)||2014|
Effect of Phoneutria nigriventer venom on the expression of junctional protein and P-gp efflux pump function in the blood-brain barrier.
13-8400 was used in western blot to use Phoneutria nigriventer spider venom to study the blood brain barrier.
|Rapôso C,Odorissi PA,Oliveira AL,Aoyama H,Ferreira CV,Verinaud L,Fontana K,Ruela-de-Sousa RR,da Cruz-Höfling MA||Neurochemical research (37:1967)||2012|
Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines.
13-8400 was used in western blot to characterize the expression and identify the cleavage fragments of Dsg2 in colonic epithelial cells.
|Kolegraff K,Nava P,Laur O,Parkos CA,Nusrat A||Cell adhesion & migration (5:306)||2011|
Histone deacetylase inhibitors enhance endothelial cell sprouting angiogenesis in vitro.
13-8400 was used in western blot to examine the effects of histone deacetylases inhibitors on capillary-like sprout formation.
|Jin G,Bausch D,Knightly T,Liu Z,Li Y,Liu B,Lu J,Chong W,Velmahos GC,Alam HB||Surgery (150:429)||2011|
Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.
13-8400 was used in immunohistochemistry to report on a case of plexiform fibromyxoma of the stomach.
|Lee PW,Yau DT,Lau PP,Chan JK||International journal of surgical pathology (22:286)||2014|
ALDH1 expression indicates chemotherapy resistance and poor outcome in node-negative rectal cancer.
13-8400 was used in immunohistochemistry to correlate ALDH expression with rectal cancer treatment response and disease outcome.
|Avoranta ST,Korkeila EA,Ristamäki RH,Syrjänen KJ,Carpén OM,Pyrhönen SO,Sundström JT||Human pathology (44:966)||2013|
Temporal and spatial localization of nectin-1 and l-afadin during synaptogenesis in hippocampal neurons.
13-8400 was used in immunocytochemistry to examine the distribution of nectin- and afadin during hippocampal synapse formation.
|Lim ST,Lim KC,Giuliano RE,Federoff HJ||The Journal of comparative neurology (507:1228)||2008|
Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus.
||Luo Y,Radice GL||Journal of cell science (116:1471)||2003|
Cell density and N-cadherin interactions regulate cell proliferation in the sensory epithelia of the inner ear.
||Warchol ME||The Journal of neuroscience : the official journal of the Society for Neuroscience (22:2607)||2002|
Expression of claudin-7 and -8 along the mouse nephron.
||Li WY,Huey CL,Yu AS||American journal of physiology. Renal physiology (286:F1063)||2004|
The role of calpain in the proteolytic cleavage of E-cadherin in prostate and mammary epithelial cells.
||Rios-Doria J,Day KC,Kuefer R,Rashid MG,Chinnaiyan AM,Rubin MA,Day ML||The Journal of biological chemistry (278:1372)||2003|
CHBCAT, Bfc, armadillo, CTNNB, Mesc, OK/SW-cl.35, Catnb, MRD19
catenin beta-1, beta-catenin, beta catenin, CATNB, CTNB1, CTNNB, catenin, beta