Table 8.5 Molecular Probes ChromaTide and aha labeled nucleotides.

Cat. No. dUTP, aha-dUTP,
or UTP Nucleotide *
Ex/Em † Applications ‡
B32766 Biotin-aha-dUTP NA RT
B32772 Biotin-aha-dCTP NA RT
Blue Fluorescence
C7612 Cascade Blue-7-dUTP 400/420 PCR, TDT, RP
Green Fluorescence
C11397 Alexa Fluor 488-5-dUTP 490/520 PCR, TDT, RP, RT, NT
C7604 Fluorescein-12-dUTP 495/525 PCR, TDT, RP, NT
C7614 BODIPY FL-14-dUTP 505/515 PCR, TDT, RP
F32767 Fluorescein-aha-dUTP 495/525 RT
C21555 Alexa Fluor 488-7-OBEA-dCTP 490/520 RT, NT
C11403 Alexa Fluor 488-5-UTP 490/520 SP6, T3, T7
Yellow Fluorescence
C11398 Alexa Fluor 532-5-dUTP 525/550 PCR, TDT, RP, RT, NT
Orange Fluorescence
C7606MP Tetramethylrhodamine-6-dUTP 550/570 PCR, TDT, RP
C11401 Alexa Fluor 546-14-dUTP 555/570 PCR, TDT, RP, RT, NT
A32762 Alexa Fluor 555-aha-dUTP 555/570 RT
A32770 Alexa Fluor 555-aha-dCTP 555/570 RT
C21556 Alexa Fluor 546-16-OBEA-dCTP 555/570 RT, NT
C11404 Alexa Fluor 546-14-UTP 555/570 SP6, T3, T7
Red Fluorescence
C11399 Alexa Fluor 568-5-dUTP 575/600 PCR, TDT, RP, NT
C7631 Texas Red-12-dUTP 595/610 PCR, TDT, RP
C11400 Alexa Fluor 594-5-dUTP 590/615 PCR, TDT, RP, NT
Far-Red Fluorescence
A32763 Alexa Fluor 647-aha-dUTP 650/670 RT
A32771 Alexa Fluor 647-aha-dCTP 650/670 RT
C21559 Alexa Fluor 647-12-OBEA-dCTP 650/670 RT, NT
* All products are supplied as either 25 µL (dUTPs and UTPs) or 50 µL (dCTPs) of a 1 mM solution in TE buffer. † Excitation (Ex) and emission (Em) maxima, in nm, for the labeled nucleotide. ‡ Except where otherwise noted, the following applications were tested: PCR, Taq polymerase for DNA amplification; TDT, terminal deoxynucleotidyl transferase for 3'-end labeling of dsDNA; RP, Klenow polymerase for labeling DNA by random hexamer priming; RT, murine leukemia virus (MLV) reverse transcriptase for synthesizing DNA from an RNA template; NT, nick translation using DNase I and DNA polymerase I. ChromaTide UTP nucleotides were incorporated into RNA by standard transcription reactions using the indicated RNA polymerases (SP6, T3, T7). We also offer unlabeled aha-dUTP (50 µL of a 50 mM solution in TE buffer, A32761) and unlabeled aha-dCTP (50 µL of a 50 mM solution in TE buffer, A32769; 500 µL of a 2 mM solution in TE buffer, A32768).