Table 2.2 Molecular Probes thiol-reactive dyes excited with ultraviolet light.

Derivative Abs * Em * Alkyl Halide or Haloacetamide Maleimide Other
Alexa Fluor 350 346 442   A30505  
Alexa Fluor 405 402 421      
Anilinonaphthalene † 326 462 IAANS, I7 MIANS, M8  
Benzophenone 282 NA   B1508  
Bimane 375 456 B30500 B30501  
Dibromobimane 394 490     bBBr, D1379 ‡
Diethylaminocoumarin 384 470 DCIA, D404 CPM, D346 ‡
MDCC, D10253
 
Dimethylaminocoumarin 376 465 DACIA, D10252 DACM, D10251 ‡  
Dimethylaminonaphthalene † 391 500 badan, B6057   acrylodan, A433
Monobromobimane 394 490     mBBr, M1378 ‡
mBBr, M20381 ‡
Monochlorobimane 394 490     mBCl, M1381MP ‡
Naphthalene † 336 490 IAEDANS, I14    
Pacific Blue 410 455   P30506  
Pacific Orange 400 551   P30507  
Phenanthroline 270 NA P6879    
Pyrene † 339 384 P29
P2007MP
P28  
Stilbene † 329 408 A484 A485  
* Approximate absorption (Abs) and emission (Em) maxima, in nm, for the reagent (if fluorescent) or the fluorescent thiol adduct. † Environment-sensitive fluorophore. ‡ Very weakly fluorescent until reacted with thiols. NA = Not applicable.