Table 19.2 Comparison of in vitro and in situ Kd values for various Ca2+ indicators.
| Indicator | Kd in vitro (nM) * | Kd in situ (nM) † | Cell/Tissue Type |
|---|---|---|---|
| Calcium Green-1 | 190 | 930 | HeLa cells  |
| Fluo-3 | 390 | 2570 | Frog skeletal muscle |
| Fluo-4 | 345 | 1000 | HeLa cells |
| Fura-2 | 145 | 371 | U373-MG astrocytoma cell |
| Fura-2 | 145 | 350 | Rabbit gastric gland |
| Indo-1 | 230 | 844 | Rabbit cardiac myocyte |
| Oregon Green 488 BAPTA-1 | 170 | 430 | HeLa cells |
| Rhod-2 | 570 | 720 | Mouse heart |
| * Values determined at 22°C in 100 mM KCl, 10 mM MOPS, pH 7.2, 0–10 mM CaEGTA. † Values determined in the cellular environments listed in the adjacent column. | |||
For Research Use Only. Not for use in diagnostic procedures.
