Table 19.2 Comparison of in vitro and in situ Kd values for various Ca2+ indicators.

Indicator Kdin vitro (nM) * Kdin situ (nM) † Cell/Tissue Type
Calcium Green-1190930HeLa cells ref
Fluo-33902570Frog skeletal muscle ref
Fluo-43451000HeLa cells ref
Fura-2145371U373-MG astrocytoma cell ref
Fura-2145350Rabbit gastric gland ref
Indo-1230844Rabbit cardiac myocyte ref
Oregon Green 488 BAPTA-1170430HeLa cells ref
Rhod-2570720Mouse heart ref
* Values determined at 22°C in 100 mM KCl, 10 mM MOPS, pH 7.2, 0–10 mM CaEGTA. † Values determined in the cellular environments listed in the adjacent column.

For Research Use Only. Not for use in diagnostic procedures.