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Table 12.3 Summary of our LysoTracker and LysoSensor probes.

Cat # Probe Abs* (nm) Em* (nm) pKa
LysoTracker Probes
L7525 LysoTracker Blue DND-22 373 422 ND
L12491 LysoTracker Yellow HCK-123 465 535 ND
L7526 LysoTracker Green DND-26 504 511 ND
L7528 LysoTracker Red DND-99 577 590 ND
L12492 LysoTracker Deep Red 647 688 ND
LysoSensor Probes
L7533 LysoSensor Blue DND-167 373 425 5.1
L7535 LysoSensor Green DND-189 443 505 5.2
L7545 LysoSensor Yellow/Blue DND-160 384
329
540 †
440 ‡
4.2
L22460 LysoSensor Yellow/Blue 10,000 MW dextran 381
335
521 †
452 ‡
3.9
* Absorption (Abs) and fluorescence emission (Em) maxima, determined in aqueous buffer or methanol; values may vary somewhat in cellular environments. † At pH 3. ‡ At pH 7; this dye has pH-dependent dual-excitation and dual-emission peaks. ND = Not determined.