Table 19.3 Parallel performance comparison of fluo-3 and fluo-4 on Molecular Devices FLIPR system.

Indicator µM * Time *(min)
Fluorescence †
Increase §
Basal Stimulated ‡
* CHO cells stably transfected with rat muscarinic M1 receptors were loaded with fluo-3 AM or fluo-4 AM according to standard protocols, with variations in indicator concentration (µM) and incubation time, as shown. † Relative fluorescence intensities after subtraction of background fluorescence. ‡ Cells were stimulated by addition of the muscarinic agonist carbachol (50 µM). § Ratio of stimulated to basal fluorescence intensities.

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