Table 6.5 Molecular Probes yellow-green–fluorescent FluoSpheres beads compared with other commercially available yellow-green–fluorescent microspheres.

Brand/Supplier
Size (µm)
Fluorescence Intensity *
CV for Intensity †
Molecular Probes F8852 1.02 1998 4.40%
Molecular Probes F8853 2.07 8998 3.26%
Company A 0.84 3.7 30.28%
Company A 1.55 5.2 11.69%
Company B 1.01 12.6 2.49%
Company B 1.94 595 2.91%
Company C 0.93 116 4.62%
Company C 1.48 434 1.92%
Company D 0.85 17 5.19%
Company D 1.84 119 3.08%
* Median value for fluorescence intensity (in arbitrary units), measured for 10,000 individual beads per sample excited at 488 nm using flow cytometry. Values may vary slightly between batches of these products. † CV = coefficient of variation.