Glycosidase Enzymes and Their Fluorogenic and Chromogenic Substrates—Table 10.1
Table 10.1 Glycosidase enzymes and their fluorogenic and chromogenic substrates.
Carbohydrate (Enzyme) |
Notes on Enzyme Activity | Labeled Substrate (Abs/Em of the products) * | Cat # | |
---|---|---|---|---|
β-D-Galactopyranoside (β-Galactosidase, E.C. 3.2.1.23) |
Blue-fluorescent product | 4-Methylumbelliferyl (360/449) ![]() |
M1489MP | |
Green-fluorescent product | Fluorescein (490/514) ![]() |
F1179 | ||
5-(Pentafluorobenzoylamino)-fluorescein (490/514) | P11948 | |||
C2-Fluorescein (490/514) ![]() |
A22010 | |||
C12-Fluorescein (490/514) ![]() |
D2893, I2904 ‡ | |||
5-Chloromethylfluorescein (490/514) ![]() |
D2920 † | |||
Red-fluorescent product | C12-Resorufin (571/585) ![]() |
I2906 ‡ | ||
DDAO (646/659) | D6488 | |||
Resorufin (571/585) ![]() |
R1159 | |||
Chromogenic substrate | 5-Bromo-4-chloro-3-indoyl (615/NA) ![]() |
X-Gal; B1690 | ||
β-D-Glucopyranoside (β-Glucosidase, E.C. 3.2.1.21) | Green-fluorescent product | Fluorescein (490/514) ![]() |
F2881 | |
5-(Pentafluorobenzoylamino)fluorescein (490/514) ![]() |
P11947 | |||
β-D-Glucuronide (β-Glucuronidase, GUS; E.C. 3.2.1.31) | Blue-fluorescent product | 4-Methylumbelliferyl (360/449) ![]() |
M1490 | |
Green-fluorescent product | Fluorescein (490/514) ![]() |
F2915 | ||
5-(Pentafluorobenzoylamino)fluorescein (490/514) ![]() |
P11949 | |||
C12-Fluorescein (490/514) ![]() |
I2908 ‡ | |||
Chromogenic substrate | 5-Bromo-4-chloro-3-indoyl (615/NA) ![]() |
X-GlcU, B1691 | ||
* Approximate absorption (Abs) and fluorescence emission (Em) maxima of enzymatic hydrolysis product, in nm. † DetectaGene Green Gene Expression Kit. ‡ ImaGene Green or ImaGene Red Gene Expression Kit. NA = Not applicable. |
For Research Use Only. Not for use in diagnostic procedures.