Table 15.4 Molecular Probes apoptosis assay kits.

Cat # Kit Name Probe(s) for Apoptotic Cells (Abs/Em) * Probe for Necrotic or Live Cells (Abs/Em) * Number of Assays Kit Features
V13240 Single Channel Annexin V/Dead Cell Apoptosis Kit Alexa Fluor 488 annexin V (495/519) SYTOX Green nucleic acid stain (504/523)

50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet)

Because this Alexa Fluor 488 annexin V–based assay uses only the green fluorescence channel on the flow cytometer, other parameters can be measured simultaneously using fluorescent probes with different emission spectra.
V13241
V13245
Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit Alexa Fluor 488 annexin V (495/519) Propidium iodide (535/617) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) Apoptotic cells are labeled with green-fluorescent Alexa Fluor 488 annexin V. Necrotic cells are labeled with red-fluorescent propidium iodide.
V13242 FITC Annexin V/Dead Cell Apoptosis Kit FITC annexin V (494/519) Propidium iodide (535/617) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) Similar to Kit V13241 except that this kit contains the fluorescein conjugate of annexin V.
V13243 Membrane Permeability/Dead Cell Apoptosis Kit YO-PRO-1 dye (491/509) Propidium iodide (535/617) 200 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) This assay detects changes in cell membrane permeability with YO-PRO-1 dye, a green-fluorescent nucleic acid stain that is permeant to apoptotic cells but not to live cells. Necrotic cells are labeled with red-fluorescent propidium iodide.
V13244 Chromatin Condensation/Dead Cell Apoptosis Kit Hoechst 33342 (346/460) Propidium iodide (535/617) 200 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) This assay uses Hoechst 33342 in combination with propidium iodide to distinguish between the condensed chromatin of apoptotic cells and the looser chromatin structure in live cells.
V23200 Vybrant Apoptosis Assay Kit #6 Biotin-X annexin V and Alexa Fluor 350 streptavidin (345/442) Propidium iodide (535/617) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet)

Apoptotic cells are labeled with the biotin-X annexin V in conjunction with blue-fluorescent Alexa Fluor 350 streptavidin. Necrotic cells are labeled with red-fluorescent propidium iodide.

V23201 Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit Hoechst 33342 (346/460) and YO-PRO-1 dye (491/509) Propidium iodide (535/617) 200 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) This kit is a combination of Kits V13243 and V13244. All three dyes can be excited by a UV laser, or by a combination of UV and 488 nm excitation.
V35112 PE Annexin V/Dead Cell Apoptosis Kit R-phycoerythrin (R-PE) annexin V (496/578) SYTOX Green nucleic acid stain (504/523) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet)

Apoptotic cells are labeled with orange-fluorescent R-phycoerythrin (R-PE) annexin V. Necrotic cells are labeled with the green-fluorescent SYTOX Green nucleic acid stain. Both probes can be excited by the 488 nm spectral line of the argon-ion laser.

V35113 APC Annexin V/Dead Cell Apoptosis Kit Allophycocyanin annexin V (650/660) SYTOX Green nucleic acid stain (504/523) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) This kit is similar to Kit V35112 except that it contains the allophycocyanin conjugate of annexin V. Apoptotic and necrotic cell populations can be distinguished using a flow cytometer equipped with an argon-ion laser and a He-Ne laser.
V35114 Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit Allophycocyanin annexin V (650/660) SYTOX Green nucleic acid stain (504/523) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) This kit is identical to Kit V35113 except that it also contains C12-resazurin, which is reduced by viable cells to the orange-fluorescent C12-resorufin. Apoptotic, necrotic and live cell populations can be distinguished using a flow cytometer equipped with both an argon-ion laser and a He-Ne laser.
V35116 Mitochondrial Membrane Potential/Annexin V Apoptosis Kit Alexa Fluor 488 annexin V (495/519) MitoTracker Red CMXRos (578/599) 50 flow cytometry assays, each containing 2 × 105 to 1 × 106 cells in a 1 mL volume (Product Information Sheet) Apoptotic cells are labeled with Alexa Fluor 488 annexin V. Live cells are labeled with MitoTracker Red CMXRos, which exhibits red fluorescence in the presence of a mitochondrial transmembrane potential. Apoptotic and live cell populations can be distinguished using a flow cytometer equipped with an argon-ion laser.
V35123 Violet Membrane Permeability/Dead Cell Apoptosis Kit PO-PRO-1 dye (435/455) 7-Aminoactinomycin D (7-AAD) (546/650) 200 flow cytometry assays, each containing 1 × 106 cells in a 1 mL volume (Product Information Sheet) This assay detects changes in cell membrane permeability with PO-PRO-1 dye, a violet-fluorescent nucleic acid stain that is permeant to apoptotic cells but not to live cells. Necrotic cells are labeled with red-fluorescent 7-AAD. Apoptotic and necrotic cell populations can be distinguished using a flow cytometer equipped with a violet diode laser and an argon-ion laser.
A35135 Violet Chromatin Condensation/Dead Cell Apoptosis Kit Vybrant DyeCycle Violet dye (370/440) SYTOX AADvanced stain (546/647) 200 flow cytometry assays, each containing 1 × 106 cells in a 1 mL volume (Product Information Sheet) This assay uses Vybrant DyeCycle Violet stain to label the condensed chromatin of apoptotic cells more brightly than the chromatin of normal cells. SYTOX AADvanced stain labels only necrotic cells, based on membrane integrity. Normal, apoptotic and necrotic cell populations can be distinguished by flow cytometry.
A35136 Violet Annexin V/Dead Cell Apoptosis Kit Pacific Blue annexin V conjugate (415/455) SYTOX AADvanced dead cell stain (546/647) 50 flow cytometry assays, each containing 1 × 106 cells in a 1 mL volume (Product Information Sheet) Apoptotic cells are labeled with the violet diode–excitable Pacific Blue annexin V. SYTOX AADvanced stain labels only necrotic cells, based on membrane integrity. Apoptotic cells show bright violet fluorescence, dead cells show red fluorescence, and live cells show dim violet fluorescence.
A35137 Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit F2N12S dye (405/530 for apoptotic and dead cells, 405/585 for live cells) SYTOX AADvanced dead cell stain (546/647) 100 flow cytometry assays, each containing 1 × 106 cells in a 1 mL volume (Product Information Sheet) This assay detects changes in cell membrane asymmetry using the ratiometric 405 nm–excitable F2N12S dye. SYTOX AADvanced stain labels only necrotic cells, based on membrane integrity. Apoptotic and necrotic cell populations can be distinguished using a flow cytometer equipped with a violet diode laser and an argon-ion laser.
* Approximate absorption and emission maxima, in nm.