Table 10.2 Rhodamine 110–based bis-peptide substrates.

Cat. No. Proteinase Substrate * Enzymes †
R22122 (Asp)2-R110 caspase-3 and other apoptosis-related proteases ref
R6506 (CBZ-Ala-Ala-Ala-Ala)2-R110 elastase ref
R33752 (CBZ-Ala-Ala-Asp)2-R110 granzyme B
R6508 (CBZ-Ala-Arg)2-R110 trypsin ref
R6501 (CBZ-Arg)2-R110 trypsin ref
R22120 (CBZ-Asp-Glu-Val-Asp)2-R110 caspase-3, -6, -7, -8, -10 ref
R6505 (CBZ-Ile-Pro-Arg)2-R110 trypsin ref
(CBZ-Ile-Glu-Thr-Asp)2-R110 caspase-8
R33753 (CBZ-Leu-Glu-Glu-Asp)2-R110 caspase-13
R6502 (CBZ-Phe-Arg)2-R110 plasmin, cathepsin L ref
R33750 (CBZ-Tyr-Val-Ala-Asp)2-R110 caspase-1, -4
R33755 (CBZ-Val-Asp-Val-Ala-Asp)2-R110 caspase-2
R33754 (CBZ-Val-Glu-Ile-Asp)2-R110 caspase-6
R22124 (p-tosyl-Gly-Pro-Arg)2-R110 thrombin ref
* CBZ = benzyloxycarbonyl; R110 = rhodamine 110; SC = succinoyl; p-tosyl = p-toluenesulfonyl. † Caspase substrates can often be cleaved by multiple enzymes. The caspase most often associated with the given peptide sequence is listed first.