Table 23.1 Spectral characteristics of Molecular Probes dyes.

Dye Excitation Maximum (nm)* Emission Maximum (nm)*
Acridine orange spectra 500 (+DNA)
460 (+RNA)
526 (+DNA)
650 (+RNA)
Alexa Fluor 350 dye, 7-amino-4-methylcoumarin (AMC) spectraspectra 346 442
Alexa Fluor 405 dye spectra 402 421
Alexa Fluor 430 dye spectra 434 539
Alexa Fluor 488 dye spectra 495 519
Alexa Fluor 514 dye spectra 518 540
Alexa Fluor 532 dye spectra 531 554
Alexa Fluor 546 dye spectra 556 573
Alexa Fluor 555 dye spectra 555 565
Alexa Fluor 568 dye spectra 578 603
Alexa Fluor 594 dye spectra 590 617
Alexa Fluor 610 dye spectra 612 628
Alexa Fluor 633 dye spectra 632 647
Alexa Fluor 635 dye spectra 633 647
Alexa Fluor 647 dye spectra 650 668
Alexa Fluor 660 dye spectra 663 690
Alexa Fluor 680 dye spectra 679 702
Alexa Fluor 700 dye spectra 702 723
Alexa Fluor 750 dye spectra 749 775
Alexa Fluor 790 dye spectra 782 805
Allophycocyanin spectra 650 660
7-Aminoactinomycin D (7-AAD) † spectra 546 647
BCECF indicator spectra 482 (low pH)
503 (high pH)
520 (low pH)
528 (high pH)
Bimane 380 458
BOBO-1, BO-PRO-1 spectraspectra 462 481
BODIPY 630/650 dye spectra 630 650
BODIPY 650/665 dye spectra 650 665
BODIPY FL dye spectraspectra 505 513
BODIPY TMR-X dye spectraspectra 542 574
BODIPY TR-X dye spectraspectra 589 617
Calcein spectra 494 517
Calcium Crimson indicator spectra 590 615
Calcium Green indicators spectra 506 533
Calcium Orange indicator spectra 549 576
Carboxy SNARF indicators spectraspectra 548 (low pH)
576 (high pH)
587 (low pH)
635 (high pH)
6-Carboxyrhodamine 6G spectra 525 555
Cascade Blue dye spectra 400 420
Cascade Yellow dye spectra 402 545
DAPI † spectra 358 461
Di-8-ANEPPS, Di-4-ANEPPS ‡spectra 468 635
DiA ‡ spectra 456 590
DiD (DiIC18(5)) spectra 644 665
DiI (DiIC18(3)) spectra 549 565
DiO (DiOC18(3)) spectra 484 501
DiR (DiIC18(7)) spectra 750 779
ELF 97 alcohol spectra 345 530
Eosin spectra 524 544
ER Tracker Blue-White DPX ‡ spectra 372 556
EthD-1 † spectra 528 617
Ethidium bromide † spectra 518 605
Fluo-3 indicator spectra 506 526
Fluo-4 indicator spectra 494 516
Fluorescein, FITC spectraspectraspectraspectra 494 518
FM 1-43, FM 1-43FX ‡ spectra 479 598
FM 4-64, FM 4-64FX ‡ spectra 506 750
Fura-2 indicator spectra 363 (low [Ca2+])
335 (high [Ca2+])
512 (low [Ca2+])
505 (high [Ca2+])
Fura Red indicator spectra 472 (low [Ca2+])
436 (high [Ca2+])
657 (low [Ca2+])
637 (high [Ca2+])
Hoechst 33258, Hoechst 33342 † spectra 352 461
7-Hydroxy-4-methylcoumarin spectra 360 455
Indo-1 indicator spectra 346 (low [Ca2+])
330 (high [Ca2+])
475 (low [Ca2+])
401 (high [Ca2+])
JC-1 spectra 498 (monomer)
593 (aggregate)
525 (monomer)
595 (aggregate)
JC-9 506 525 (monomer)
635 (aggregate)
JOE dye spectra 525 555
Lissamine rhodamine B 570 590
Lucifer yellow CH spectra 428 536
LysoSensor Blue DND-167 spectra 374 425
LysoSensor Green DND-153, DND-189 spectra 442 505
LysoSensor Yellow/Blue DND-160 (PDMPO) spectra 384 (low pH)
329 (high pH)
540 (low pH)
440 (high pH)
LysoTracker Green spectra 504 511
LysoTracker Red spectra 577 592
Magnesium Green indicator spectra 506 531
Marina Blue dye spectra 365 460
MitoTracker Green FM spectra 490 516
MitoTracker Orange CMTMRos spectra 551 576
MitoTracker Red CMXRos spectra 578 599
Monobromobimane § spectra 394 490
NBD amines spectra 465 535
NeuroTrace 500/525 green-fluorescent Nissl stain † spectra 500 525
Nile red ‡ spectra 549 628
Oregon Green 488 dye and Oregon Green 488 BAPTA indicators spectra 496 524
Oregon Green 514 dye spectra 511 530
Pacific Blue dye spectra 410 455
Pacific Orange dye spectra 400 551
POPO-1, PO-PRO-1 † spectraspectra 434 456
Propidium iodide † spectra 536 617
Rhodamine 110 spectra 496 520
Rhodamine Red dye spectra 570 590
R-phycoerythrin spectra 565 575
Resorufin spectra 571 585
RH 414 ‡ 500 635
Rhod-2 indicator spectra 552 578
Rhodamine Green dye spectra 502 527
Rhodamine 123 spectra 507 529
ROX dye spectra 580 605
Sodium Green indicator spectra 507 535
SYTO blue-fluorescent nucleic acid stains 40, 41, 42, 43 † 428 ± 6 454 ± 13
SYTO blue-fluorescent nucleic acid stains 44, 45 † spectra 450 ± 5 478 ± 7
SYTO green-fluorescent nucleic acid stains 12, 13, 16, 21, 23, 24 † spectra 494 ± 6 515 ± 7
SYTO green-fluorescent nucleic acid stains 11, 14, 15, 20, 22, 25 † spectra 515 ± 7 543 ± 13
SYTO orange-fluorescent nucleic acid stains 80, 81, 82, 83 † 537 ± 7 552 ± 8
SYTO orange-fluorescent nucleic acid stains 84, 85 † 567 583
SYTO red-fluorescent nucleic acid stains 17, 59, 61, 64 † spectra 615 ± 15 632 ± 13
SYTO red-fluorescent nucleic acid stains 60, 62, 63 † 655 ± 3 675 ± 3
SYTOX Blue nucleic acid stain † spectra 445 470
SYTOX Green nucleic acid stain † spectra 504 523
SYTOX Orange nucleic acid stain † spectra 547 570
Tetramethylrhodamine, Rhodamine B spectraspectraspectraspectra 555 580
Texas Red and Texas Red-X dye spectraspectra 595 615
TOTO-1, TO-PRO-1 † spectraspectra 514 533
TOTO-3, TO-PRO-3 † spectraspectra 642 660
X-rhod-1 indicator spectra 580 602
YOYO-1, YO-PRO-1 † spectraspectra 491 509
YOYO-3, YO-PRO-3 † spectraspectra 612 631
* Fluorescence excitation and emission maxima in nm are listed here, and fluorescence spectra are available at (www.lifetech.com/spectraviewer). Transmittance curves and other specific microscopy filter information can be found through microscope manufacturers or at filter manufacturer websites, including Chroma Technology Corp. (www.chroma.com), Omega Optical, Inc. (www.omegafilters.com) and  Semrock, Inc. (www.semrock.com). † Excitation and emission peaks listed for dye/nucleic acid complex. ‡ Excitation and emission peaks listed for lipid-bound dye. § Excitation and emission peaks listed for glutathione-conjugated dye.