Table 2.1 Molecular Probes thiol-reactive dyes excited with visible light.

Derivative Abs * Em * Maleimide Haloacetamide Bromomethyl Halide Cystine C or Thiosulfate T
Alexa Fluor 488 495 519 A10254 M        
Alexa Fluor 532 532 553 A10255        
Alexa Fluor 546 556 575 A10258 M        
Alexa Fluor 555 555 565 A20346        
Alexa Fluor 568 578 603 A20341 M        
Alexa Fluor 594 590 617 A10256 M        
Alexa Fluor 633 632 647 A20342 M        
Alexa Fluor 647 650 665 A20347        
Alexa Fluor 660 663 690 A20343        
Alexa Fluor 680 679 702 A20344        
Alexa Fluor 750 749 775 A30459        
BODIPY FL 505 513 B10250 D6003     B20340 C
T30453 T
BODIPY TMR 542 574 B30466       T30454 T
BODIPY TR 589 617         T30455 T
BODIPY 493/503 493 503     B2103    
BODIPY 499/508 499 508 D20350        
BODIPY 507/545 508 543   D6004      
BODIPY 577/618 577 618 D20351        
BODIPY 630/650 625 640     B22802   T30456 T
4-Dimethylamino phenylazophenyl 419 NA D1521        
Eosin 524 544 E118 5        
Fluorescein 494 518 F150 5 I30451 5
I30452 6
B1355 5    
Lucifer yellow 426 531   L1338      
NBD 478 541   I9 †
D2004
 

F486
F6053 ‡
C20260

 
Oregon Green 488 496 524 O6034 5 O6010 M      
PyMPO 415 570 M6026        
QSY 7 560 NA Q10257        
QSY 9 562 NA Q30457        
QSY 35 475 NA   Q20348      
Rhodamine Red 570 590 R6029 M        
Sulfonerhodamine 555 580   B10621 §      
Tetramethylrhodamine 555 580 T6027 5
T6028 6
T6006 5      
Texas Red 595 615 T6008 M   T6009 M    
* Absorption (Abs) and emission (Em) maxima, in nm. † Iodoacetate ester. ‡ Like the NBD probes, ABD-F (F6053) is a benz-2-oxa-1,3-diazole, except that it is sulfonated (i.e., an SBD probe) instead of nitrated (i.e., an NBD probe); its reaction product with dimethylaminoethanethiol has Abs/Em maxima of 376/510 nm. § Bifunctional crosslinker. 5 = 5-Isomer. 6 = 6-Isomer. M = Mixed isomers. C = BODIPY FL L-cystine. T = TS-Link fluorescent thiosulfate. NA = Not applicable.