Table 18.2 Scavengers of reactive oxygen species (ROS).
|ROS||Scavenger (Working Concentration)||References|
|Hydrogen peroxide (H2O2)||Sodium pyruvate (10 mM), DMTU * (10 mM)|
|Hydroxyl radical (HO•)||Mannitol (20–100 mM), DMSO (0.28 M) †|
|Nitric oxide (NO)||Carboxy-PTIO (C7912; 100 µM)|
|Peroxyl radical (ROO•)||Trolox ‡ (10–100 µM), α-tocopherol (10–100 µM)|
|Peroxynitrite anion (ONOO–)||Ebselen § (10–100 µM), uric acid (100 µM)|
|Singlet oxygen (1O2)||Sodium azide (1–10 mM)|
|Superoxide anion (•O2–)||MnTBAP ** (100 µM), Tiron †† (10 mM)|
|* DMTU = N,N'-dimethylthiourea. Disproportionation by catalase is also widely used for suppression of H2O2.
† 0.28 M DMSO = 2% (v/v). The reactivity of OH• is so high that it can be argued that the actions of these reagents must be indirect.
‡ Trolox = 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.
§ Ebselen = 2-phenyl-1,2-benzisoselenazol-3(2H)-one.
** MnTBAP = manganese(III)-tetrakis(4-benzoic acid)porphyrin.
†† Tiron = 4,5-dihydroxybenzene-1,3-disulfonate.
For Research Use Only. Not for use in diagnostic procedures.