Table 4.1 Biotinylation and desthiobiotinylation reagents.

Reactive Moiety Biotin Derivative (Cat. No.) DSB-X Biotin Derivative (Cat. No.)
Aliphatic amine A1593, A1594, B1596 (XX), N6356  
Alkyne B10185  
Azide B10184  
Carboxylic acid B1595, B20656, B1592 D20657
DNP-X–biocytin-X, SE B2604, F6348 (F)  
Hydrazide B1603, B2600 (XX) D20653 (X)
Hydroxylamine A10550  
Iodoacetamide B1591 D30753
Maleimide M1602  
Succinimidyl ester (SE) B1513, B1582 (X), B1606 (XX), B6353 (SSE)(X), B6352 (SSE)(XX), A10495 (APEX), B30010 (Micro), B30756 (Micro), F2610 (F), F6347 (F) D20655 (D)
TS-Link thiosulfate   T30754
(APEX) = APEX Antibody Labeling Kit. (D) = DSB-X Biotin Protein Labeling Kit. (F) = FluoReporter Protein Labeling Kit. (Micro) = Biotin-XX Microscale Protein Labeling Kit. (SE) = Succinimidyl ester. (SSE) = Sulfosuccinimidyl ester. (X) = Aminohexanoyl (7-atom) spacer separating the biotin or desthiobiotin and the reactive moiety. (XX) = Aminohexanoylaminohexanoyl (14-atom) spacer separating the biotin and the reactive moiety.