Invitrogen™

eBioscience™ 7-AAD Viability Staining Solution

Catalog number:  00-6993-50

Related applications:

Flow Cytometry

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Description

7-AAD (7-amino-actinomycin D) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 7-AAD can be used in place of PI (propidium iodide). This solution can be used in combination with PE (phycoerythrin) and FITC (fluorescein isothiocyanate) conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability: Cell-Impermeant
Color: Red
Detection Method: Fluorescent
Excitation⁄Emission (nm): 535⁄617
For Use With (Equipment): Flow Cytometer
Product Line: eBioscience™
Product Size: 2 mL