Mounting Media and Antifades

Preserve the fluorescence signal from your sample

Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity, particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration. The mechanisms associated with photobleaching are also implicated in phototoxicity, which may adversely impact cell viability and data quality.

To minimize photobleaching of experimental samples, we have developed a series of antifade reagents that increase fluorophore photostability in both live-cell and fixed-cell samples. We offer choices for immediate imaging and for long-term storage, plus formulations that include a nuclear stain to combine mounting and counterstaining in a single step. Reduced photobleaching means longer tracking for time-course experiments, higher sensitivity, and better quantitation from fluorescence signals.

Select the antifade that matches your dye or fluorescent protein

 
What type of imaging am I planning?
Live-cell imaging
       
Fixed-cell, immediate imaging      
Fixed-cell, long-term imaging  
   
What fluorophores does my sample include?
Alexa Fluor™ dyes
Traditional dyes
 
 
Fluorescent proteins
 
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI  
No counterstain

Green chemistry

green chemistry

 

For added convenience and a reduction in the environmental impact associated with cold storage requirements, our antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks. Learn more about these green chemistry initiatives

For Research Use Only. Not for use in diagnostic procedures.