Mounting Media and Antifades

Preserve the fluorescent signal from your sample

Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity, particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration. The mechanisms associated with photobleaching are also implicated in phototoxicity, which may adversely impact cell viability and data quality.

To minimize photobleaching of experimental samples, we have developed a series of antifade reagents that increase fluorophore photostability in both live-cell and fixed-cell samples. We offer choices for immediate imaging and for long-term storage, plus formulations that include a nuclear stain to combine mounting and counterstaining in a single step. Reduced photobleaching means longer tracking for time-course experiments, higher sensitivity, and better quantitation from fluorescent signals.

Select the antifade that matches your fluorescence imaging needs

  ProLong Glass ProLong Diamond ProLong Gold
  Learn more
General specifications
Form Ready-to-use hard-setting mountant
Refractive index 1.52 (after curing) 1.47 (after curing) 1.47 (after curing)
What type of imaging am I planning?
Specimen type Animal cells/tissue (fixed)
Live-cell imaging      
Fixed-cell, immediate imaging      
Fixed-cell, long-term imaging (up to 10 µm sample thickness)
Fixed-cell, long-term imaging (up to 150 µm sample thickness)
   
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
   
Glycerol-corrected objective  
Water- or air-corrected objective      
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
No counterstain
  SlowFade Glass SlowFade Diamond SlowFade Gold
  Learn more
General specifications
Form Ready-to-use soft-setting mountant
Refractive index 1.52 1.42 1.42
What type of imaging am I planning?
Specimen type Animal cells/tissue (fixed)
Live-cell imaging      
Fixed-cell, immediate imaging
Fixed-cell, long-term imaging (up to 10 µm sample thickness)      
Fixed-cell, long-term imaging (up to 500 µm sample thickness)
   
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective      
Glycerol-corrected objective
Water- or air-corrected objective      
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
 
   
No counterstain
  ProLong Live
  Learn more
General specifications
Form 100x concentrated liquid
Refractive index 1.3
What type of imaging am I planning?
Specimen type Animal cells/tissue (live)
Live-cell imaging
Fixed-cell, immediate imaging  
Fixed-cell, long-term imaging (up to 10 µm sample thickness)  
Fixed-cell, long-term imaging (up to 150 µm sample thickness)  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective  
Glycerol-corrected objective
Water- or air-corrected objective
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red  
No counterstain
  ProLong Glass
(curing)
SlowFade Glass
(non-curing)
CytoVista Reagents
(non-curing)
  Learn more Learn more Learn more
General Specifications
Form Ready-to-use, curing (hard-setting) mountant

For immediate imaging of samples up to 150 mm thick
Ready-to-use non-curing (soft-set) mountant

For immediate imaging of samples up to 500 mm thick
Ready-to-use, non-curing (soft-setting) family of reagents

3D Cell Culture clearing and staining kit for immediate imaging of 3D cell culture (organoids and spheroids up to 1 mm thick)

Tissue clearing and staining kit for immediate imaging of thick tissue (up to 10 mm)
Refractive index 1.52 (after curing) 1.52 1.48 (organoids/spheroids up to 1 mm thick)

1.53 (tissue samples up to 10 mm thick)
What type of imaging am I planning?
Specimen type Animal cells/tissue (fixed) Animal cells/tissue (fixed) Animal cells/tissue (fixed)
Fixed-cell imaging, immediate imaging  
Fixed-cell, long-term imaging
   
What is the thickness of my sample?
Up to 10 μm
   
Up to 150 μm
   
Up to 500 μm  
 
Up to 1 mm    
Up to 10 mm    
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
Glycerol-corrected objective    
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red
   
No counterstain
  Image-iT Plant Clearing and Mounting Reagents for Imaging
  Learn more
General Specifications
Form Ready-to-use reagents for clearing and mounting plant tissue for imaging
Refractive index 1.45 (clearing and soft-set mounting option)

1.415 (hard-set mounting option)
What type of imaging am I planning?
Specimen type Plant cells/tissue section/whole tissue (fixed)
Live-cell imaging  
Fixed-cell imaging
Fixed-cell imaging, long-term imaging (up to 10 μM sample thickness)
Fixed-cell imaging, long-term imaging (up to 150 μM sample thickness)
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective  
Glycerol-corrected objective
Water- or air-corrected objective  
What fluorophores does my sample include?
Most common histological stains
Fluorescent proteins  
Do I want to add counterstain in the mountant?
Counterstain with DAPI  
Counterstain with SYTOX Deep Red  
No counterstain
  ProLong Glass ProLong Diamond ProLong Gold
  Learn more
General specifications
Form Ready-to-use hard-setting mountant
Refractive index 1.52 (after curing) 1.47 (after curing) 1.47 (after curing)
What type of imaging am I planning?
Specimen type Animal cells/tissue (fixed)
Live-cell imaging      
Fixed-cell, immediate imaging      
Fixed-cell, long-term imaging (up to 10 µm sample thickness)
Fixed-cell, long-term imaging (up to 150 µm sample thickness)
   
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
   
Glycerol-corrected objective  
Water- or air-corrected objective      
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
No counterstain
  SlowFade Glass SlowFade Diamond SlowFade Gold
  Learn more
General specifications
Form Ready-to-use soft-setting mountant
Refractive index 1.52 1.42 1.42
What type of imaging am I planning?
Specimen type Animal cells/tissue (fixed)
Live-cell imaging      
Fixed-cell, immediate imaging
Fixed-cell, long-term imaging (up to 10 µm sample thickness)      
Fixed-cell, long-term imaging (up to 500 µm sample thickness)
   
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective      
Glycerol-corrected objective
Water- or air-corrected objective      
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
 
   
No counterstain
  ProLong Live
  Learn more
General specifications
Form 100x concentrated liquid
Refractive index 1.3
What type of imaging am I planning?
Specimen type Animal cells/tissue (live)
Live-cell imaging
Fixed-cell, immediate imaging  
Fixed-cell, long-term imaging (up to 10 µm sample thickness)  
Fixed-cell, long-term imaging (up to 150 µm sample thickness)  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective  
Glycerol-corrected objective
Water- or air-corrected objective
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red  
No counterstain
  ProLong Glass
(curing)
SlowFade Glass
(non-curing)
CytoVista Reagents
(non-curing)
  Learn more Learn more Learn more
General Specifications
Form Ready-to-use, curing (hard-setting) mountant

For immediate imaging of samples up to 150 mm thick
Ready-to-use non-curing (soft-set) mountant

For immediate imaging of samples up to 500 mm thick
Ready-to-use, non-curing (soft-setting) family of reagents

3D Cell Culture clearing and staining kit for immediate imaging of 3D cell culture (organoids and spheroids up to 1 mm thick)

Tissue clearing and staining kit for immediate imaging of thick tissue (up to 10 mm)
Refractive index 1.52 (after curing) 1.52 1.48 (organoids/spheroids up to 1 mm thick)

1.53 (tissue samples up to 10 mm thick)
What type of imaging am I planning?
Specimen type Animal cells/tissue (fixed) Animal cells/tissue (fixed) Animal cells/tissue (fixed)
Fixed-cell imaging, immediate imaging  
Fixed-cell, long-term imaging
   
What is the thickness of my sample?
Up to 10 μm
   
Up to 150 μm
   
Up to 500 μm  
 
Up to 1 mm    
Up to 10 mm    
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
Glycerol-corrected objective    
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red
   
No counterstain
  Image-iT Plant Clearing and Mounting Reagents for Imaging
  Learn more
General Specifications
Form Ready-to-use reagents for clearing and mounting plant tissue for imaging
Refractive index 1.45 (clearing and soft-set mounting option)

1.415 (hard-set mounting option)
What type of imaging am I planning?
Specimen type Plant cells/tissue section/whole tissue (fixed)
Live-cell imaging  
Fixed-cell imaging
Fixed-cell imaging, long-term imaging (up to 10 μM sample thickness)
Fixed-cell imaging, long-term imaging (up to 150 μM sample thickness)
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective  
Glycerol-corrected objective
Water- or air-corrected objective  
What fluorophores does my sample include?
Most common histological stains
Fluorescent proteins  
Do I want to add counterstain in the mountant?
Counterstain with DAPI  
Counterstain with SYTOX Deep Red  
No counterstain

Greener products from Thermo Fisher Scientific

For added convenience and a reduction in the environmental impact associated with cold storage requirements, our antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks.

Learn more about these greener product alternatives

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