Mounting Media and Antifades

Preserve the fluorescent signal from your sample

Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity, particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration. The mechanisms associated with photobleaching are also implicated in phototoxicity, which may adversely impact cell viability and data quality.

To minimize photobleaching of experimental samples, we have developed a series of antifade reagents that increase fluorophore photostability in both live-cell and fixed-cell samples. We offer choices for immediate imaging and for long-term storage, plus formulations that include a nuclear stain to combine mounting and counterstaining in a single step. Reduced photobleaching means longer tracking for time-course experiments, higher sensitivity, and better quantitation from fluorescent signals.

Select the antifade that matches your fluorescence imaging needs

 ProLong GlassProLong DiamondProLong Gold
 Learn more
General specifications
FormReady-to-use hard-setting mountant
Refractive index1.52 (after curing)1.47 (after curing)1.47 (after curing)
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (fixed)
Live-cell imaging   
Fixed-cell, immediate imaging   
Fixed-cell, long-term imaging (up to 10 µm sample thickness)
Fixed-cell, long-term imaging (up to 150 µm sample thickness)
  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
  
Glycerol-corrected objective 
Water- or air-corrected objective   
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
No counterstain
 SlowFade GlassSlowFade DiamondSlowFade Gold
 Learn more
General specifications
FormReady-to-use soft-setting mountant
Refractive index1.521.421.42
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (fixed)
Live-cell imaging   
Fixed-cell, immediate imaging
Fixed-cell, long-term imaging (up to 10 µm sample thickness)   
Fixed-cell, long-term imaging (up to 500 µm sample thickness)
  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective   
Glycerol-corrected objective
Water- or air-corrected objective   
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
 
  
No counterstain
 ProLong Live
 Learn more
General specifications
Form100x concentrated liquid
Refractive index1.3
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (live)
Live-cell imaging
Fixed-cell, immediate imaging 
Fixed-cell, long-term imaging (up to 10 µm sample thickness) 
Fixed-cell, long-term imaging (up to 150 µm sample thickness) 
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective 
Glycerol-corrected objective
Water- or air-corrected objective
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red 
No counterstain
 ProLong Glass
(curing)
SlowFade Glass
(non-curing)
CytoVista Reagents
(non-curing)
 Learn moreLearn moreLearn more
General Specifications
FormReady-to-use, curing (hard-setting) mountant

For immediate imaging of samples up to 150 mm thick
Ready-to-use non-curing (soft-set) mountant

For immediate imaging of samples up to 500 mm thick
Ready-to-use, non-curing (soft-setting) family of reagents

3D Cell Culture clearing and staining kit for immediate imaging of 3D cell culture (organoids and spheroids up to 1 mm thick)

Tissue clearing and staining kit for immediate imaging of thick tissue (up to 10 mm)
Refractive index1.52 (after curing)1.521.48 (organoids/spheroids up to 1 mm thick)

1.53 (tissue samples up to 10 mm thick)
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (fixed)Animal cells/tissue (fixed)Animal cells/tissue (fixed)
Fixed-cell imaging, immediate imaging 
Fixed-cell, long-term imaging
  
What is the thickness of my sample?
Up to 10 μm
  
Up to 150 μm
  
Up to 500 μm 
 
Up to 1 mm  
Up to 10 mm  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
Glycerol-corrected objective  
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red
  
No counterstain
 Image-iT Plant Clearing and Mounting Reagents for Imaging
 Learn more
General Specifications
FormReady-to-use reagents for clearing and mounting plant tissue for imaging
Refractive index1.45 (clearing and soft-set mounting option)

1.415 (hard-set mounting option)
What type of imaging am I planning?
Specimen typePlant cells/tissue section/whole tissue (fixed)
Live-cell imaging 
Fixed-cell imaging
Fixed-cell imaging, long-term imaging (up to 10 μM sample thickness)
Fixed-cell imaging, long-term imaging (up to 150 μM sample thickness)
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective 
Glycerol-corrected objective
Water- or air-corrected objective 
What fluorophores does my sample include?
Most common histological stains
Fluorescent proteins 
Do I want to add counterstain in the mountant?
Counterstain with DAPI 
Counterstain with SYTOX Deep Red 
No counterstain
 ProLong GlassProLong DiamondProLong Gold
 Learn more
General specifications
FormReady-to-use hard-setting mountant
Refractive index1.52 (after curing)1.47 (after curing)1.47 (after curing)
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (fixed)
Live-cell imaging   
Fixed-cell, immediate imaging   
Fixed-cell, long-term imaging (up to 10 µm sample thickness)
Fixed-cell, long-term imaging (up to 150 µm sample thickness)
  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
  
Glycerol-corrected objective 
Water- or air-corrected objective   
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
No counterstain
 SlowFade GlassSlowFade DiamondSlowFade Gold
 Learn more
General specifications
FormReady-to-use soft-setting mountant
Refractive index1.521.421.42
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (fixed)
Live-cell imaging   
Fixed-cell, immediate imaging
Fixed-cell, long-term imaging (up to 10 µm sample thickness)   
Fixed-cell, long-term imaging (up to 500 µm sample thickness)
  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective   
Glycerol-corrected objective
Water- or air-corrected objective   
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
 
Fluorescent proteins
 
Do I want to add counterstain in the mountant?
Counterstain with DAPI
Counterstain with SYTOX Deep Red
 
  
No counterstain
 ProLong Live
 Learn more
General specifications
Form100x concentrated liquid
Refractive index1.3
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (live)
Live-cell imaging
Fixed-cell, immediate imaging 
Fixed-cell, long-term imaging (up to 10 µm sample thickness) 
Fixed-cell, long-term imaging (up to 150 µm sample thickness) 
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective 
Glycerol-corrected objective
Water- or air-corrected objective
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red 
No counterstain
 ProLong Glass
(curing)
SlowFade Glass
(non-curing)
CytoVista Reagents
(non-curing)
 Learn moreLearn moreLearn more
General Specifications
FormReady-to-use, curing (hard-setting) mountant

For immediate imaging of samples up to 150 mm thick
Ready-to-use non-curing (soft-set) mountant

For immediate imaging of samples up to 500 mm thick
Ready-to-use, non-curing (soft-setting) family of reagents

3D Cell Culture clearing and staining kit for immediate imaging of 3D cell culture (organoids and spheroids up to 1 mm thick)

Tissue clearing and staining kit for immediate imaging of thick tissue (up to 10 mm)
Refractive index1.52 (after curing)1.521.48 (organoids/spheroids up to 1 mm thick)

1.53 (tissue samples up to 10 mm thick)
What type of imaging am I planning?
Specimen typeAnimal cells/tissue (fixed)Animal cells/tissue (fixed)Animal cells/tissue (fixed)
Fixed-cell imaging, immediate imaging 
Fixed-cell, long-term imaging
  
What is the thickness of my sample?
Up to 10 μm
  
Up to 150 μm
  
Up to 500 μm 
 
Up to 1 mm  
Up to 10 mm  
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
Glycerol-corrected objective  
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
 
Counterstain with SYTOX Deep Red
  
No counterstain
 Image-iT Plant Clearing and Mounting Reagents for Imaging
 Learn more
General Specifications
FormReady-to-use reagents for clearing and mounting plant tissue for imaging
Refractive index1.45 (clearing and soft-set mounting option)

1.415 (hard-set mounting option)
What type of imaging am I planning?
Specimen typePlant cells/tissue section/whole tissue (fixed)
Live-cell imaging 
Fixed-cell imaging
Fixed-cell imaging, long-term imaging (up to 10 μM sample thickness)
Fixed-cell imaging, long-term imaging (up to 150 μM sample thickness)
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective 
Glycerol-corrected objective
Water- or air-corrected objective 
What fluorophores does my sample include?
Most common histological stains
Fluorescent proteins 
Do I want to add counterstain in the mountant?
Counterstain with DAPI 
Counterstain with SYTOX Deep Red 
No counterstain

For Research Use Only. Not for use in diagnostic procedures.