Mounting Media and Antifades

Preserve the fluorescent signal from your sample

Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity, particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration. The mechanisms associated with photobleaching are also implicated in phototoxicity, which may adversely impact cell viability and data quality.

To minimize photobleaching of experimental samples, we have developed a series of antifade reagents that increase fluorophore photostability in both live-cell and fixed-cell samples. We offer choices for immediate imaging and for long-term storage, plus formulations that include a nuclear stain to combine mounting and counterstaining in a single step. Reduced photobleaching means longer tracking for time-course experiments, higher sensitivity, and better quantitation from fluorescent signals.

Select the antifade that matches your fluorescence imaging needs

	ProLong Glass	ProLong Diamond	ProLong Gold	ProLong Live	SlowFade Diamond	SlowFade Gold
	Learn more			Learn more	Learn more
General specifications
Form	Ready-to-use hard-setting mountant			100x concentrated liquid	Ready-to-use soft-setting mountant
Refractive index	1.52 (after curing)	1.47 (after curing)	1.47 (after curing)	1.3	1.42	1.42
What type of imaging am I planning?
Live-cell imaging
Fixed-cell, immediate imaging
Fixed-cell, long-term imaging (up to 10 µm sample thickness)
Fixed-cell, long-term imaging (up to 150 µm sample thickness)
What type of microscope objective can produce best results? (All objective types are compatible with these reagents)
Oil immersion objective
Glycerol-corrected objective
Water- or air-corrected objective
What fluorophores does my sample include?
Alexa Fluor dyes
Traditional dyes
Fluorescent proteins
Do I want to add counterstain in the mountant?
Counterstain with DAPI
No counterstain

Greener products from Thermo Fisher Scientific

image representing Thermo Fisher Scientific's greener products initiative

For added convenience and a reduction in the environmental impact associated with cold storage requirements, our antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks.

Learn more about these greener product alternatives