Qdot® Label Conjugates for Cell & Tissue Staining
Detecting low abundance antigens with even the best conventional dye conjugates can be a challenge, as photobleaching can make it difficult to effectively observe and record staining. The exceptional photostability of Qdot® Nanocrystal conjugates can provide substantial benefits in the detection of low abundance targets. Due to their narrow and symmetric emission spectra, Qdot® nanocrystals are also ideal for multicolor, multiplexed fluorescence detection using a single excitation source such as the 405 nm laser.
Qdot® Streptavidin Conjugates
Qdot® streptavidin conjugates combine the unsurpassed photostability of Qdot® nanocrystals with the highly specific binding properties of streptavidin. These powerful fluorescence detection reagents offer unique performance advantages in a wide variety of tissue labeling and flow cytometry experiments; they are efficiently excited using the 405 nm violet laser, and the Qdot® Nanocrystal fluorescence is extremely resistant to photobleaching.
Qdot® Streptavidin Conjugate contents
These products provide 200 µL of the Qdot® Streptavidin conjugate and a detailed protocol outlining the use of the product in immunohistochemistry (IHC) applications using fluorescence microscopy. At conventional working concentrations, ~100 staining experiments may be performed
Qdot® Strepavidin Conjugate Sampler Kit contents
Each Qdot® Streptavidin Sampler Kit provides 50 µL of each of six colors of Qdot® streptavidin conjugate (525, 565, 585, 605, 655, and 705 nm emission) and a detailed protocol describing the use of the product in immunohistochemistry (IHC) applications using fluorescence microscopy. At conventional working concentrations, ~20 staining experiments with each color may be performed.
Qdot® Secondary Antibody Conjugates
Qdot® secondary antibody conjugates are ideal immunoreagents for use in fluorescence microscopy, microplate-based assays, flow cytometry, and many other applications. These conjugates are made with the F(ab')2 fragment of affinity-purified goat antibodies, cross-adsorbed against the serum proteins of numerous species. Qdot secondary antibody conjugates can be used in standard physiological buffers with BSA present from pH 4–9 in a variety of high-salt conditions, to achieve high-quality staining with incredibly low background.
Qdot® Secondary Antibody Conjugate contents
Each kit is supplied with 200 µL of the Qdot secondary antibody conjugate and a protocol describing the use of the product in immunohistochemistry applications using fluorescence microscopy. At conventional working concentrations, approximately 100 staining experiments may be performed.
Qdot® Conjugated Primary Antibodies and WGA
Fusion tags such as glutathione-s-transferase (GST) can greatly simplify purification and detection of proteins that are not yet well-characterized or do not elicit a strong immune response to produce antibodies. Our Qdot® 655 anti-glutathione-s-transferase antibody conjugate can be used to detect GST-tagged proteins in western blotting, fluorescence microscopy or microtiter plate based applications.
In addition to being widely used as a fluorochrome, fluorescein is an excellent hapten that can be recognized by secondary detection reagents in a variety of applications. Many primary or secondary detection reagents, such as nucleic acid probes and antibodies, can be effectively linked to fluorescein and subsequently detected by Qdot® anti-fluorescein conjugates. Anti-fluorescein conjugates also provide an alternative to a traditional biotin–avidin system. Essentially all of the methods that use biotin and avidin reagents are amenable to the fluorescein–anti- fluorescein system, which helps avoid the background caused by endogenous biotin in cells and tissues in some applications. Also available is the Qdot® 655 rat anti-dinitrophenol conjugate for detection of the hapten, dinitrophenol (DPH).
Wheat germ agglutinin is a 36,000 molecular weight protein that binds to N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids. There are many uses for WGA conjugates including cell surface labeling and measuring retrograde neuronal transport. Our Qdot® 655 wheat germ agglutinin conjugate provides highly sensitive labeling of N-acetylglucosamine residues, with low non-specific binding.
Qdot® primary conjugate product contents
Each unit is supplied with 200 µL of the Qdot® conjugate and a protocol describing the use of the product in fluorescence microscopy applications.
We also have an extensive array of Qdot® primary antibodies that are validated for flow cytometry.
Qdot® Antibody Conjugation Kits
Qdot® antibody conjugation kits allow you to conjugate your antibodies to Qdot® nanocrystals. Kits are available in a wide range of colors—525, 565, 605 655, 705 or 800 nm emission—and the procedure can be completed in a few hours. The conjugation method is based on the fast and efficient coupling of thiols to maleimide groups.
Qdot® antibody conjugates generated with these kits are ideally suited for applications such as immunohistochemistry, tissue staining, ELISA, Western blotting, flow cytometry, and other user-designed experiments. In addition to conjugation of antibodies, other thiol-containing molecules can be coupled to Qdot® nanocrystals using this kit.
Qdot® Antibody Conjugation Kits contents
The kits contain all necessary reagents and components for two conjugation reactions, each using 300 µg of an IgG antibody, as well as a detailed protocol.