Invitrogen

CD47 Monoclonal Antibody (B6H12), FITC, eBioscience™

Name: CD47 Monoclonal Antibody (B6H12), FITC, eBioscience\u2122
Brand: Invitrogen
SKU: 11-0479-42

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23 Published Figures

22 References

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Additional Information:

FIGURE: 1 / 25

CD47 Antibody (11-0479-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (blue histogram) or CD47 Monoclonal Antibody, FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD47 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD47 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD47 Antibody in Immunohistochemistry (IHC)

CD47 Antibody in Inhibition Assays (Inhibition)

Product Image Gallery

CD47 Antibody — Figure 8 GMP-grade iExosomes stability. ( A ) Comparison of the number of MSC exosomes, quantified by NanoSight, prior to freezing and after thawing of frozen exosomes ( n = 4 distinct exosomes aliquots). The times listed refer to the times that exosomes were stored for at -80degC. ( B ) Particle size distribution analysis of fresh and frozen (45 days) and then thawed MSC exosomes by NanoSight. ( C ) TEM of MSC exosomes, prior to freezing (fresh) and after freezing (45 days) and thawing. Scale bar: 100 nm. ( D ) Representative histogram of flow cytometry analyses of exosomal markers (CD9, CD63, CD81, CD47) on fresh versus freeze (45 days) and thaw MSC exosomes. Numbers represent the percentage of positive beads (gray, isotype control). ( E and F ) Representative dot plot of flow cytometry analyses ( E ) and quantification ( F ) of apoptosis in Panc-1 cells induced by MSCs siKras G12D iExo (48 hours following iExo treatment), comparing the efficacy of freeze (3 months) and thaw iExosomes that were allowed to incubate for 3, 6, and 24 hours and 2, 3, 4, and 5 days at room temperature (RT) or 4degC ( n = 2-3 independent experiments). Numbers represent the percentage of positive cells. One-way ANOVA compared with fresh exosomes. ( G ) KRAS G12D transcript levels in Panc-1 cells treated with MSCs siKras G12D iExo after 3 hours, comparing the efficacy of freeze (3 months) and thaw of iExosomes, under the listed conditions ( n = 3 independent experiments, 1 {TM}

Product Details

11-0479-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 15 publications 15 publications

Inhibition Assays (Inhibition)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B6H12

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2043842

Product Specific Information

Description: The monoclonal antibody B6H12 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Recently stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity also increasing expression of Foxp3. Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

This antibody has been reported to have neutralizing activity.

Applications Reported: This B6H12 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This B6H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47; unnamed protein product

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Gene Aliases: CD47; IAP; MER6; OA3

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UniProt ID: (Human) Q08722

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Entrez Gene ID: (Human) 961

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Function(s)

protein binding fibrinogen binding thrombospondin receptor activity protein binding involved in heterotypic cell-cell adhesion protein binding involved in cell-cell adhesion

Process(es)

angiogenesis positive regulation of immune system process apoptotic process inflammatory response cell adhesion integrin-mediated signaling pathway positive regulation of cell proliferation cell migration positive regulation of cell-cell adhesion regulation of interferon-gamma production regulation of interleukin-10 production regulation of interleukin-12 production regulation of interleukin-6 production regulation of tumor necrosis factor production heterotypic cell-cell adhesion regulation of nitric oxide biosynthetic process positive regulation of inflammatory response negative regulation of phagocytosis positive regulation of phagocytosis positive regulation of T cell activation positive regulation of stress fiber assembly regulation of Fc receptor mediated stimulatory signaling pathway cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-12 cell-cell adhesion ATP export positive regulation of monocyte extravasation

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CD47 Monoclonal Antibody (B6H12), FITC, eBioscience™

CD47 Antibody (11-0479-42) in Flow

Product Details

11-0479-42

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Inhibition Assays (Inhibition)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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