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CD150 Monoclonal Antibody (A12 (7D4)), FITC, eBioscience™
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FIGURE: 1 / 2
CD150 Antibody (11-1509-42) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD19 PE-Cy7 and CD150 FITC. As expected based on known relative expression patterns, CD150 clone A12 (7D4) stains monocytes (orange), granulocytes (blue) and a subset of lymphocytes (pink).
Product Details
11-1509-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
A12 (7D4)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells.
Applications Reported: This A12 (7D4) antibody has been reported for use in flow cytometric analysis.
Applications Tested: The A12 (7D4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg)/test per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD150; CD150 antigen; CDw150; cSLAM; IPO-3; Signaling lymphocytic activation molecule; SLAM family member 1; unnamed protein product
Gene Aliases: CD150; CDw150; IPO3; SLAM; SLAMF1
UniProt ID: (Human) Q13291
Entrez Gene ID: (Human) 6504
natural killer cell differentiation
natural killer cell proliferation
negative regulation of cytokine production
leukocyte chemotaxis involved in inflammatory response
adaptive immune response
myeloid dendritic cell activation involved in immune response
immune system process
negative regulation of T cell cytokine production
phagocytosis
immune response
cell adhesion
positive regulation of cell proliferation
positive regulation of macrophage chemotaxis
regulation of vesicle fusion
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interferon-gamma production
positive regulation of activated T cell proliferation
innate immune response
leukocyte activation
positive regulation of JNK cascade
lymphocyte activation
viral entry into host cell
positive regulation of ERK1 and ERK2 cascade
negative regulation of CD40 signaling pathway
positive regulation of dendritic cell chemotaxis
positive regulation of T-helper 1 cell cytokine production
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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