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Invitrogen
CD205 Monoclonal Antibody (MG38), FITC, eBioscience™
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FIGURE: 1 / 3
CD205 Antibody (11-2059-82) in Flow
Normal human peripheral blood cells were stained with 0.015 µg Mouse IgG2b kappa Isotype Control, FITC (Product # 11-4732-81) (blue histogram) or 0.015 µg CD205 Monoclonal Antibody, FITC (purple histogram). Cells in the monocyte gate were used for analysis.
Product Details
11-2059-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MG38
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8^+ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.
Applications Reported: The MG38 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood cells. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region. Further, CD205 also prompts the reduced proliferation of B lymphocytes. Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: C-type lectin domain family 13 member B; CA-IX; CD205; DCL-1 fusion; DEC-205; gp200; gp200-MR6; Ly-75; Lymphocyte antigen 75; membrane antigen MN; P54/58N; pMW1; RP23-10L17.4; similar to Homo sapiens DEC-205
Gene Aliases: CD205; CLEC13B; DEC-205; GP200-MR6; LY-75; LY75
UniProt ID: (Human) O60449
Entrez Gene ID: (Human) 4065
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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