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Invitrogen
Rat anti-Mouse IgG (H+L) Secondary Antibody, FITC, eBioscience™
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FIGURE: 1 / 10
Mouse IgG (H+L) Secondary Antibody (11-4011-85) in Flow
Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 PE (Product # 12-0441-82), followed by 0.125 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 0.125 µg of Anti-Phycoerythrin Purified (right), followed by detection with Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.
Product Details
11-4011-85
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
1:4000
Flow Cytometry (Flow)
0.5 µg/test
Product Specifications
Host/Isotype
Rat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
500 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Description: The rat polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reaction to human, bovine, horse, rat and rabbit serum proteins. Reactions to non-immunoglobulin mouse serum proteins have not been detected. This antibody will recognize the light chains of other mouse immunoglobulins.
Applications Reported: The polyclonal antibody has been reported for use in detection of purified mouse immunoglobulins (IgG) in flow cytometry.
Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis to detect purified mouse monoclonal antibodies. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse, rat and rabbit serum proteins, but may cross-react with immunoglobµLins from other species.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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