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Invitrogen
CD32b Monoclonal Antibody (AT130-2), PE, eBioscience™
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FIGURE: 1 / 2
CD32b Antibody (12-0321-82) in Flow
Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or 0.25 µg of Anti-Mouse CD32b PE (right). Total viable cells were used for analysis.
Product Details
12-0321-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AT130-2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody AT130-2 reacts with mouse CD32b, also known as Fc-gamma receptor IIb. CD32b is the only inhibitory member of the Fc-gamma receptor family. Immune complex engagement of CD32b results in the phosphorylation of its intracellular ITIM motif. This engagement serves to attenuate B cell receptor (BCR) signaling and other activating signals, reduces proliferation, reduces antibody production, and may also deliver apoptotic signals. CD32b is the only Fc-gamma receptor expressed by B cells. CD32b is also expressed on monocytes, dendritic cells, neutrophils, eosinophils and basophils, as well as B cell progenitors and plasma cells.
AT130-2 has been reported to have agonist activity.
Applications Reported: This AT130-2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AT130-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD32; CD32 receptor 2; CD32 receptor 2a; CD32 receptor 2b; CD32A; Fc gamma receptor IIB; Fc gamma RIIB; Fc-gamma RII; fc-gamma RII-a; Fc-gamma-RII; fc-gamma-RIIa; fc-gamma-RIIB; FcgammaRIIB; FcGR; FCGR2A1; fcRII-a; FGFR2B; IgG Fc receptor II beta; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; Lymphocyte antigen 17
Gene Aliases: AI528646; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1
UniProt ID: (Mouse) P08101
Entrez Gene ID: (Mouse) 14130
negative regulation of type I hypersensitivity
immune complex clearance
negative regulation of immunoglobulin production
negative regulation of inflammatory response to antigenic stimulus
negative regulation of acute inflammatory response to antigenic stimulus
negative regulation of humoral immune response mediated by circulating immunoglobulin
receptor-mediated endocytosis
phagocytosis, engulfment
defense response
immune response
cell surface receptor signaling pathway
response to bacterium
immunoglobulin mediated immune response
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of B cell proliferation
endocytic recycling
negative regulation of interleukin-10 production
negative regulation of neutrophil apoptotic process
intracellular signal transduction
mast cell activation
negative regulation of phagocytosis
positive regulation of phagocytosis
negative regulation of immune response
cellular response to molecule of bacterial origin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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