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Invitrogen
Integrin beta 5 Monoclonal Antibody (KN52), PE, eBioscience™
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Product Details
12-0497-41
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
KN52
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets.
The KN52 monoclonal antibody has been shown to inhibit migration.
Applications Reported: This KN52 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This KN52 antibody has been pre-titrated and tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD61; FLJ26658; GP3A; GPIIIa; Integrin beta-5; integrin beta-5 subunit; integrin beta-5 subunit precursor; integrin, beta 5; testis secretory sperm-binding protein Li 217p; unnamed protein product
Gene Aliases: [b]-5; [b]5; [b]5A; [b]5B; AA475909; AI874634; beta-5; beta5; ESTM23; I79_000655; ITGB5
UniProt ID: (Human) P18084, (Mouse) O70309
Entrez Gene ID: (Human) 3693, (Mouse) 16419, (Chinese hamster) 100765378
formation of primary germ layer
cell adhesion
cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
cell migration
cell adhesion mediated by integrin
wound healing, spreading of epidermal cells
endodermal cell differentiation
stress fiber assembly
viral entry into host cell
epithelial cell-cell adhesion
cell-cell adhesion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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