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CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), PE, eBioscience™
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FIGURE: 1 / 23
CD62P (P-Selectin) Antibody (12-0626-82) in Flow
Staining of Thrombin-activated human platelets with Anti-Human CD41a FITC (Product # 11-0419-42) and 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Human/Mouse CD62P (P-Selectin) PE (right). Total viable cells were used for analysis.
Product Details
12-0626-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Psel.KO2.3
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat) and has not been reported to block cell adhesion.
Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Psel.KO2.3 antibody was tested by flow cytometric analysis on thrombin-activated human platelets. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granule membrane protein-140 (GMP-140) precursor; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa antigen CD62) isoform SELP_1; selectin P (granule membrane protein 140kDa antigen CD62) isoform SELP_3; selectin P (granule membrane protein 140kDa, antigen CD62); unnamed protein product
Gene Aliases: CD62; CD62P; GMP-140; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP
UniProt ID: (Human) P16109, (Mouse) Q01102
Entrez Gene ID: (Human) 6403, (Mouse) 20344
positive regulation of leukocyte migration
regulation of cellular extravasation
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
positive regulation of platelet activation
positive regulation of phosphatidylinositol 3-kinase signaling
single organismal cell-cell adhesion
regulation of integrin activation
positive regulation of cell adhesion
leukocyte migration
leukocyte tethering or rolling
cellular response to interleukin-6
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
response to lipopolysaccharide
response to cytokine
defense response to Gram-negative bacterium
positive regulation of protein kinase B signaling
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of leukocyte tethering or rolling
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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