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Invitrogen
CD74 Monoclonal Antibody (5-329), PE, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-0748-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5-329
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 5-329 monoclonal antibody recognizes human CD74, also known as the MHC Class II-associated invariant chain. A 33-43 kDa nonpolymorphic type II membrane protein, CD74 is highly expressed on B cells and subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells. Monocytes and macrophages also express CD74, but at lower levels. CD74 acts as a chaperone of MHC Class II (HLA-DR) proteins, promoting their translocation from the ER to endocytic compartments during antigen presentation. Activation of NFkappaB and ERK has been shown following CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells. CD74 has also been demonstrated to interact with CXCR2 and CXCR4.
Applications Reported: This 5-329 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5-329 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; class II MHC-associated invariant chain peptide; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia-associated invariant chain; MHC HLA-DR gamma chain; unnamed protein product
Gene Aliases: CLIP; DHLAG; HLADG; Ia-GAMMA; II; p33
UniProt ID: (Human) P04233
Entrez Gene ID: (Human) 972
beta-amyloid binding
cytokine receptor activity
protein binding
cytokine binding
MHC class II protein complex binding
macrophage migration inhibitory factor binding
MHC class II protein binding
CD4 receptor binding
MHC class II protein binding, via antigen binding groove
identical protein binding
protein binding involved in protein folding
nitric-oxide synthase binding
prostaglandin biosynthetic process
positive regulation of cytokine-mediated signaling pathway
T cell activation involved in immune response
positive regulation of dendritic cell antigen processing and presentation
negative regulation of peptide secretion
positive regulation of type 2 immune response
negative regulation of mature B cell apoptotic process
protein folding
intracellular protein transport
immune response
positive regulation of gene expression
immunoglobulin mediated immune response
antigen processing and presentation
antigen processing and presentation of endogenous antigen
negative regulation of cell migration
positive regulation of B cell proliferation
positive regulation of prostaglandin biosynthetic process
positive regulation of chemokine production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
response to interferon-gamma
macrophage migration inhibitory factor signaling pathway
regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
negative regulation of DNA damage response, signal transduction by p53 class mediator
T cell selection
positive regulation of monocyte differentiation
negative regulation of viral entry into host cell
positive regulation of fibroblast proliferation
protein stabilization
positive regulation of macrophage cytokine production
protein-containing complex assembly
protein trimerization
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
positive regulation of macrophage migration inhibitory factor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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