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CD135 (Flt3) Monoclonal Antibody (A2F10), PE, eBioscience™
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FIGURE: 1 / 6
CD135 (Flt3) Antibody (12-1351-82) in Flow
Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 APC (Product # 17-1171-82) and 0.5 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.5 µg of Anti-Mouse CD135 (Flt3) PE (right). Cells were also stained with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) and Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), and viable, lineage negative/low cells in the large scatter population were used for analysis.
Product Details
12-1351-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
A2F10
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.
Applications Reported: The A2F10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2
Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl
UniProt ID: (Mouse) Q00342
Entrez Gene ID: (Mouse) 14255
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
cytokine receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
ubiquitin protein ligase binding
protein complex binding
glucocorticoid receptor binding
phosphatidylinositol 3-kinase binding
transmembrane signal receptor
leukocyte homeostasis
positive regulation of protein phosphorylation
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
pro-B cell differentiation
pro-T cell differentiation
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
negative regulation of cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
hemopoiesis
lymphocyte differentiation
cell differentiation
B cell differentiation
common myeloid progenitor cell proliferation
negative regulation of B cell differentiation
lymphocyte proliferation
protein autophosphorylation
cellular response to cytokine stimulus
dendritic cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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