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Invitrogen
CD166 (ALCAM) Monoclonal Antibody (3A6), PE, eBioscience™
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Product Details
12-1668-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Deer,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
3A6
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 3A6 monoclonal antibody reacts with human CD166, which is also known as activated leukocyte cell adhesion molecule (ALCAM). CD166 is an immunoglobulin superfamily cell adhesion molecule that is broadly expressed on developing and adult hematopoietic (e.g., activated T and B cells, monocytes, and dendritic cells) and non-hematopoietic (e.g., neural, endothelial, and epithelial) tissues. CD166 has also been detected in melanoma, colorectal carcinoma, as well as cancers of the breast, prostate, and bladder. CD166 mediates cell-cell adhesion by forming low-affinity homophilic interactions (ALCAM-ALCAM) or high-affinity heterophilic interactions with its ligand CD6 (ALCAM-CD6). CD166 plays a role in development, inflammation, and neutrophil migration. Moreover, its expression can be used as a prognostic marker for several cancers and has been reported to be a cancer stem cell marker.
The 3A6 antibody has been reported to induce the internalization of CD166 and its recycling back to the cell surface.
Applications Reported: This 3A6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3A6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Activated leukocyte cell adhesion molecule; ALCAM/CD166; CD166; CD166 antigen; FLJ38514; MGC71733; unnamed protein product
Gene Aliases: ALCAM; CD166; MEMD
UniProt ID: (Human) Q13740
Entrez Gene ID: (Human) 214
adaptive immune response
immune system process
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
signal transduction
axon guidance
motor neuron axon guidance
retinal ganglion cell axon guidance
axon extension involved in axon guidance
neuron projection extension
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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