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Invitrogen
MICA/B Monoclonal Antibody (6D4), PE, eBioscience™
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Product Details
12-5788-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
6D4
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.
Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: cell stress-regulated membrane protein; HLA class I antigen; ligand to the NKG2D receptor; major histocompatibility complex class I chain-related protein A; MHC class I antigen-related protein B; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related chain A; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MIC-A; MIC-B; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; unnamed protein product
Gene Aliases: MIC-A; MICA; MICB; PERB11.1; PERB11.2
UniProt ID: (Human) Q95IY7, (Human) Q29980
Entrez Gene ID: (Human) 100507436, (Human) 4277
T cell mediated cytotoxicity
adaptive immune response
immune system process
immune response to tumor cell
immune response
cellular response to DNA damage stimulus
signal transduction
response to heat
negative regulation of defense response
killing of cells of other organism
negative regulation of natural killer cell activation
natural killer cell mediated cytotoxicity
defense response to bacterium
negative regulation of natural killer cell mediated cytotoxicity
gamma-delta T cell activation
regulation of immune response
defense response to virus
immune response-activating cell surface receptor signaling pathway
response to oxidative stress
response to retinoic acid
negative regulation of defense response to virus by host
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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