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Invitrogen

CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), PE, eBioscience™

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4 Published Figures
15 References
View all (36) OX40L (CD252) antibodies

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Product Details

12-5905-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
View 15 publications 15 publications
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RM134L

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466036

Product Specific Information

Description: The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.

Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: atherosclerosis 1; CD252; OX40 ligand; OX40L; tax-transcriptionally activated glycoprotein 1 ligand; Tumor necrosis factor ligand superfamily member 4

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Gene Aliases: Ath-1; Ath1; CD134L; gp34; OX-40L; Ox40l; Tnfsf4; TXGP1; Txgp1l

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UniProt ID: (Mouse) P43488

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Entrez Gene ID: (Mouse) 22164

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Function(s)
cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding
Process(es)
blood vessel development cytokine production defense response to nematode acute inflammatory response positive regulation of immune effector process positive regulation of T cell cytokine production regulation of adaptive immune response positive regulation of type 2 immune response positive regulation of immunoglobulin mediated immune response inflammatory response immune response cholesterol metabolic process response to virus negative regulation of interferon-gamma production negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production memory T cell activation T-helper 2 cell activation response to nitrogen dioxide cellular response to nitrogen dioxide CD4-positive, alpha-beta T cell costimulation T cell proliferation positive regulation of activated T cell proliferation positive regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of memory T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity innate immune response negative regulation of regulatory T cell differentiation negative regulation of T-helper 1 cell differentiation positive regulation of T-helper 2 cell differentiation negative regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation negative regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of B cell activation positive regulation of immunoglobulin secretion negative regulation of T cell apoptotic process negative regulation of activation-induced cell death of T cells cellular response to lipopolysaccharide cellular response to prostaglandin E stimulus chemokine (C-C motif) ligand 11 production positive regulation of CD4-positive, alpha-beta T cell costimulation positive regulation of T cell migration positive regulation of T cell costimulation positive regulation of memory T cell activation positive regulation of T-helper 2 cell activation positive regulation of interleukin-4-dependent isotype switching to IgE isotypes negative regulation of extrinsic apoptotic signaling pathway
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