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CX3CR1 Monoclonal Antibody (2A9-1), PE, eBioscience™
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FIGURE: 1 / 4
CX3CR1 Antibody (12-6099-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or Anti-Human CX3CR1 PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-6099-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2A9-1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.
Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2A9-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G-protein coupled receptor 13; Scyd1; unnamed protein product; V28
Gene Aliases: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; V28
UniProt ID: (Human) P49238
Entrez Gene ID: (Human) 1524
positive regulation of neuroblast proliferation
adaptive immune response
microglial cell activation involved in immune response
immune system process
response to ischemia
chemotaxis
inflammatory response
immune response
cellular defense response
cell adhesion
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
brain development
response to wounding
negative regulation of angiogenesis
cytokine-mediated signaling pathway
calcium-mediated signaling
central nervous system maturation
leukocyte chemotaxis
regulation of tumor necrosis factor production
negative regulation of interleukin-1 beta production
social behavior
autocrine signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of nitric oxide biosynthetic process
regulation of synaptic plasticity
host-mediated regulation of intestinal microbiota composition
regulation of neurogenesis
positive regulation of neurogenesis
modulation of synaptic transmission
leukocyte tethering or rolling
positive regulation of protein kinase B signaling
synapse maturation
cell chemotaxis
antifungal innate immune response
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to cytokine stimulus
positive regulation of monocyte chemotaxis
synapse pruning
negative regulation of hippocampal neuron apoptotic process
multiple spine synapse organization, single dendrite
regulation of microglial cell migration
negative regulation of microglial cell mediated cytotoxicity
negative regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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