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CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), PE, eBioscience™
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FIGURE: 1 / 1
CCL2 (MCP-1) Antibody (12-7099-80) in Flow
Normal human peripheral blood cells were stimulated with LPS for 5 hours in the presence of Monensin Solution (Product # 00-4505-51). The cells were intracellularly stained with Anti-Human CD11c PerCP-eFluor® 710 (Product # 46-0116-42) and 0.06 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.06 µg of Anti-Human CCL2 (MCP-1) PE (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.
Product Details
12-7099-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5D3-F7
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
Applications Reported: This 5D3-F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 5D3-F7 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; monocyte chemotactic protein; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Mutations: 171:OK; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2; unnamed protein product
Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
UniProt ID: (Human) P13500
Entrez Gene ID: (Human) 6347
angiogenesis
monocyte chemotaxis
protein phosphorylation
chemotaxis
inflammatory response
immune response
humoral immune response
cytoskeleton organization
cell adhesion
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
JAK-STAT cascade
regulation of cell shape
response to bacterium
organ morphogenesis
positive regulation of gene expression
positive regulation of macrophage chemotaxis
viral genome replication
cytokine-mediated signaling pathway
sensory perception of pain
cellular homeostasis
positive regulation of cell migration
negative regulation of glial cell apoptotic process
helper T cell extravasation
chemokine (C-C motif) ligand 2 signaling pathway
negative regulation of neuron apoptotic process
astrocyte cell migration
cellular response to fibroblast growth factor stimulus
eosinophil chemotaxis
macrophage chemotaxis
positive regulation of T cell activation
positive chemotaxis
positive regulation of synaptic transmission, glutamatergic
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of calcium ion import
positive regulation of glutamate receptor signaling pathway
negative regulation of vascular endothelial cell proliferation
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of endothelial cell apoptotic process
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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