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Invitrogen

CD289 (TLR9) Monoclonal Antibody (eB72-1665), PE, eBioscience™

1 Published Figure
16 References
View all (39) TLR9 antibodies

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Product Details

12-9099-82

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test
View 14 publications 14 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Pig

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eB72-1665

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466239

Product Specific Information

Description: eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy (Nat Immunol. 5:190). Human Epithelial Cell lines were also reported to stain with this mAb (J. Immunol. 173: 1219). Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

Applications Reported: This eB72-1665 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This eB72-1665 antibody has been tested by intracellular flow cytometric analysis of hTLR9 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD289; orphan receptor; Toll-like receptor 9; unspliced isoform

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Gene Aliases: CD289; TLR9; UNQ5798/PRO19605

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UniProt ID: (Human) Q9NR96

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Entrez Gene ID: (Human) 54106

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Function(s)
interleukin-1 receptor binding siRNA binding pattern recognition receptor activity protein homodimerization activity unmethylated CpG binding
Process(es)
microglial cell activation positive regulation of cytokine production activation of innate immune response toll-like receptor signaling pathway immune system process positive regulation of immunoglobulin production regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway inflammatory response immune response signal transduction I-kappaB kinase/NF-kappaB signaling male gonad development response to virus positive regulation of autophagy positive regulation of gene expression maintenance of gastrointestinal epithelium positive regulation of B cell proliferation positive regulation of type I interferon production detection of molecule of bacterial origin positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production toll-like receptor 9 signaling pathway positive regulation of toll-like receptor 9 signaling pathway defense response to bacterium positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade innate immune response regulation of B cell differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade regulation of inflammatory response positive regulation of inflammatory response defense response to Gram-negative bacterium regulation of B cell activation positive regulation of B cell activation defense response to virus negative regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to metal ion defense response to other organism endolysosomal toll-like receptor signaling pathway negative regulation of calcium-transporting ATPase activity cellular response to chloroquine positive regulation of intestinal epithelial cell development
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