Search
Invitrogen
EVA1 Monoclonal Antibody (G9P3-1), PE, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
EVA1 Antibody (12-9742-82) in Flow
C57BL/6 thymocytes were stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8 FITC (Product # 11-0081-82), Anti-Human/Mouse CD44 PE-eFluor® 610 (Product # 61-0441-82), Anti-Mouse CD25 PerCP-Cyanine5-5 (Product # 45-0251-82), and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human/Mouse EVA1 PE. Isotype control (blue histogram) or EVA1 (purple histogram) staining on DN3 cells (CD4-CD8-CD44-CD25+) are shown and compared to EVA1 staining on DN1 cells (CD4-CD8-CD44+CD25-) (orange histogram). Viable cel... View More
Product Details
12-9742-82
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
G9P3-1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody G9P3-1 reacts with human and mouse epithelial V-like antigen 1 (EVA1), also known as myelin protein zero-like 2 (MPZL2). EVA1 is a low affinity adhesion type I transmembrane glycoprotein that bears an immunolobulin V-type domain. This glycoprotein is expressed on some epithelial tissues and also on immature thymocytes in the DN3 stage. The DN3 thymocytes are characterized by CD4 and CD8 double negative cells that are CD25 positive and CD44 negative. EVA1 has a role in immature thymocyte development, and thymic regeneration.
Applications Reported: This G9P3-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This G9P3-1 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. This gene is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cell adhesion molecule; Epithelial V-like antigen 1; immunoglobulin-like protein; Myelin protein zero-like protein 2; unnamed protein product
Gene Aliases: DFNB111; EVA; EVA1; MPZL2; UNQ606/PRO1192
UniProt ID: (Human) O60487
Entrez Gene ID: (Human) 10205, (Mouse) 14012
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support