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          • Primary Antibodies ›
          • LAP Antibodies

          Invitrogen

          LAP (Latency Associated peptide) Monoclonal Antibody (TW7-16B4), PE, eBioscience™

          1 Published Figure
          2 References
          View all (12) LAP antibodies

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          Cite LAP (Latency Associated peptide) Monoclonal Antibody (TW7-16B4), PE, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          LAP (Latency Associated peptide) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          LAP (Latency Associated peptide) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          LAP (Latency Associated peptide) Antibody (12-9821-82) in Flow

          BALB/c splenocytes were stimulated with Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) and Mouse IL-2 Recombinant Protein (Product # 14-8021-64) for 1 day. Cells were surface stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of An... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          LAP (Latency Associated peptide) Antibody in Flow Cytometry (Flow)
          LAP (Latency Associated peptide) Antibody in Flow Cytometry (Flow)

          Product Details

          12-9821-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.25 µg/test
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TW7-16B4

          Conjugate

          PE PE PE
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572734

          Product Specific Information

          Description: The TW7-16B4 monoclonal antibody reacts with mouse latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells.

          Applications Reported: This TW7-16B4 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This TW7-16B4 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: regulatory protein; TGF-beta 1; TGF-beta-1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor-beta 1

          View more View less

          Gene Aliases: TGF-beta1; Tgfb; Tgfb-1; Tgfb1; TGFbeta1

          View more View less

          UniProt ID: (Mouse) P04202

          View more View less

          Entrez Gene ID: (Mouse) 21803

          View more View less

          Function(s)
          glycoprotein binding antigen binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding protein homodimerization activity protein heterodimerization activity protein N-terminus binding growth factor
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade skeletal system development morphogenesis of a branching structure cell activation epithelial to mesenchymal transition negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis defense response inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly SMAD protein import into nucleus Notch signaling pathway negative regulation of neuroblast proliferation endoderm development skeletal muscle tissue development negative regulation of DNA replication cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration defense response to fungus, incompatible interaction organ morphogenesis response to organic substance regulation of gene expression positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production cell growth regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus negative regulation of cell-cell adhesion hyaluronan catabolic process T cell differentiation negative regulation of ossification negative regulation of cell growth positive regulation of cell migration mammary gland development positive regulation of histone deacetylation organ regeneration positive regulation of protein complex assembly positive regulation of exit from mitosis lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production receptor catabolic process regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of histone acetylation positive regulation of protein dephosphorylation growth wound healing T cell activation regulation of cell proliferation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis myelination regulation of apoptotic process myeloid dendritic cell differentiation T cell homeostasis positive regulation of apoptotic process positive regulation of MAP kinase activity regulation of MAPK cascade protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity regulatory T cell differentiation cell-cell junction organization negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation positive regulation of isotype switching to IgA isotypes cell development lymph node development negative regulation of skeletal muscle tissue development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion negative regulation of phagocytosis negative regulation of immune response negative regulation of T cell activation positive regulation of chemotaxis positive regulation of NF-kappaB transcription factor activity regulation of binding regulation of DNA binding positive regulation of smooth muscle cell differentiation negative regulation of release of sequestered calcium ion into cytosol positive regulation of cell division positive regulation of protein kinase B signaling pathway-restricted SMAD protein phosphorylation positive regulation of SMAD protein import into nucleus SMAD protein signal transduction mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis negative regulation of gene silencing by miRNA regulation of cartilage development lens fiber cell differentiation response to cholesterol positive regulation of cell cycle arrest cellular response to growth factor stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus T-helper 17 cell lineage commitment extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of protein localization to nucleus positive regulation of NAD+ ADP-ribosyltransferase activity regulation of actin cytoskeleton reorganization positive regulation of transcription regulatory region DNA binding
          It has to be done as per old AB suggested Products section.

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