Search
Invitrogen
Blimp-1 Monoclonal Antibody (5E7), PE, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Blimp-1 Antibody (12-9850-80) in Flow
Splenocytes isolated from sheep red blood cell-immunized mice were stained with Fixable Viability Dye eFluor® 506 (Product # 65-0866-14) to exclude dead cells from analysis. Then the cells were intracellularly stained with Anti-Mouse Bcl-6 PerCP-eFluor® 710 (Product # 46-5453-82) and 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse Blimp1 PE (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells expressing CD11c, F4/80, Gr-1, CD4, and CD8 w... View More
Product Details
12-9850-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5E7
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1. Blimp-1 is a transcriptional repressor with five zinc finger domains. Blimp-1 is expressed by terminally differentiating B and T cells. Blimp-1 is also expressed by some dendritic cells, and some NK cells. This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation. While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation. Blimp-1 promotes effector molecule secretion while suppressing proliferation. Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation.
Applications Reported: The 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: The 5E7 antibody has been tested by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: B lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; BLIMP 1; Blimp-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; RP1-134E15.1
Gene Aliases: b2b1765Clo; Blimp-1; Blimp1; PRDI-BF1; Prdm1; ZNFPR1A1
UniProt ID: (Mouse) Q60636
Entrez Gene ID: (Mouse) 12142
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support