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CD45RA Monoclonal Antibody (JS-83), PE, eBioscience™
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FIGURE: 1 / 4
CD45RA Antibody (12-9979-41) in Flow
Knockout of CD45RA was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR664203_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD45RA was performed by staining THP-1 CD45RA Knock out cells with 0.03 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82) or 0.03 µg CD45RA Monoclonal Antibody (JS-83), PE, eBioscience™ (Product # 12-9979-42, blue histogram). THP-1 Cas9 control cells were also stained with0.03 µg CD45... View More
Product Details
12-9979-41
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
JS-83
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The JS-83 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4^+ peripheral T lymphocytes, CD8^+ peripheral T lymphocytes, and B cells. The CD45RA^+ T cell populations are mainly naive/virgin allowing the use of JS-83 mAb as a phenotypic marker to discriminate T cell subsets.
Applications Reported: JS-83 has been reported for use in flow cytometric analysis.
Applications Tested: This JS-83 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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