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Invitrogen
CD34 Monoclonal Antibody (4H11), Biotin, eBioscience™
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FIGURE: 1 / 60
CD34 Antibody (13-0349-82) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD45 APC (Product # 17-0459-42) and 0.25 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 0.25 µg of Anti-Human CD34 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Product Details
13-0349-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
4H11
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.
Applications Reported: This 4H11 (APG) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4H11 (APG) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD34; CD34 antigen; CD34 sialomucin; glycoprotein expressed in lymphohematopoietic progenitor cells; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2; unnamed protein product
Gene Aliases: CD34
UniProt ID: (Human) P28906
Entrez Gene ID: (Human) 947
tissue homeostasis
endothelial cell proliferation
glomerular filtration
endothelium development
cell adhesion
cell-matrix adhesion
signal transduction
positive regulation of gene expression
negative regulation of gene expression
hemopoiesis
negative regulation of interleukin-2 production
mesangial cell-matrix adhesion
paracrine signaling
positive regulation of odontogenesis
positive regulation of angiogenesis
cell motility
leukocyte migration
transdifferentiation
vascular wound healing
hematopoietic stem cell proliferation
positive regulation of granulocyte colony-stimulating factor production
extracellular exosome assembly
glomerular endothelium development
stem cell proliferation
metanephric glomerular mesangial cell differentiation
cell-cell adhesion
positive regulation of vasculogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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