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Invitrogen
CD68 Monoclonal Antibody (815CU17), Biotin, eBioscience™
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FIGURE: 1 / 1
CD68 Antibody (13-0687-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 10 µg/mL of Mouse IgG1 K Isotype Control Biotin (left) or 10 µg/mL of Anti-Human CD68 Biotin (right), followed by Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
13-0687-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
815CU17
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 815CU17 monoclonal antibody reacts with human CD68, a 110 kDa, transmembrane glycoprotein that belongs to the sialomucin family and is closely related to the lysosomal-associated membrane proteins (LAMP) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of mainly myeloid-derived cells: monocytes, macrophages, dendritic cells (and in some langerhans cells), neutrophils, basophils, mast cells, myeloid progenitor cells, and a subset of CD34+ hematopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on homology and structure, CD68 may play a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also shown expression in activated T cells, and about 40% of peripheral blood B lymphocytes and 50% of all B-ALL.
This 815CU17 antibody does not cross-react with mouse or rat CD68.
For flow cytometric analysis of human CD68, we recommend clone eBioY1/82A.
Applications Reported: This 815CU17 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This 815CU17 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 110kda transmembrane glycoprotein; CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1; unnamed protein product
Gene Aliases: CD68; GP110; LAMP4; SCARD1
UniProt ID: (Human) P34810
Entrez Gene ID: (Human) 968
inflammatory response to antigenic stimulus
negative regulation of dendritic cell antigen processing and presentation
cellular response to nutrient levels
autocrine signaling
response to ethanol
cellular response to lipopolysaccharide
establishment of protein localization to organelle
cellular response to oxidised low-density lipoprotein particle stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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