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Invitrogen
Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), Biotin, eBioscience™
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FIGURE: 1 / 1
Ly-49D Antibody (13-5782-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.25 µg of Rat IgG2a kappa Isotype Control Biotin (Product # 13-4321-82) (left) or 0.25 µg of Anti-Mouse Ly-49D Biotin (right) followed by Streptavidin APC (Product # 17-4314-82). Total viable cells were used for analysis.
Product Details
13-5782-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio4E5 (4E5)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R. eBio4E5 is reported to have functional (activating) activity.
Applications Reported: This eBio4E5 (4E5) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio4E5 (4E5) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Killer cell lectin-like receptor 4; killer cell lectin-like receptor subfamily A member 32; killer cell lectin-like receptor subfamily A member 33; Ly-49d; Lymphocyte antigen 49d; T-cell surface glycoprotein Ly-49D
Gene Aliases: Chok; Klra32; Klra33; Klra4; Ly-49d; Ly49-d; Ly49d; ly49r<129>
UniProt ID: (Mouse) Q60651
Entrez Gene ID: (Mouse) 16635
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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