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Invitrogen
P-cadherin Monoclonal Antibody (NCC-CAD-299)
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FIGURE: 1 / 2
P-cadherin Antibody (13-5800) in Neu
The Journal of pathology 2008 -
6 Modulation of RT4 growth on collagen after 5 days. (A) RT4 growth without antibody (negative experimental control). (B) RT4 growth in the presence of anti-CK20 antibody (negative antibody control). (C) RT4 growth in the presence of anti-P-cadherin function-blocking antibody. (A-C) All show RT4 forming tight rounded multicellular colonies without any noticeable morphological changes. (D) RT4 growth in the ... View More
Product Details
13-5800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
NCC-CAD-299
Immunogen
Epidermoid carcinoma cell line A431.
Conjugate
Form
Lyophilized
Concentration
2.0 mg/mL
Amount
100 µg
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with human epidermal carcinoma cell A431.1) The monoclonal antibody was harvested from ascitic fluid.
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL).This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Specificity: This antibody specifically reacts with human P-cadherin. This antibody inhibits P-cadherin dependent cell-cell contact.
Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
Target Information
P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CADH3; cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; P-cadherin; Placental cadherin; Placental-cadherin; unnamed protein product
Gene Aliases: CDH3; CDHP; HJMD; PCAD
UniProt ID: (Human) P22223
Entrez Gene ID: (Human) 1001
cell morphogenesis
retina homeostasis
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
visual perception
positive regulation of gene expression
positive regulation of keratinocyte proliferation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
hair cycle process
negative regulation of transforming growth factor beta receptor signaling pathway
keratinization
positive regulation of monophenol monooxygenase activity
adherens junction organization
positive regulation of insulin-like growth factor receptor signaling pathway
cell-cell adhesion mediated by cadherin
positive regulation of melanin biosynthetic process
regulation of transport
negative regulation of catagen
positive regulation of canonical Wnt signaling pathway
cell-cell adhesion
positive regulation of melanosome transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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