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Invitrogen
CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), Biotin, eBioscience™
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FIGURE: 1 / 6
CD254 (RANK Ligand) Antibody (13-5952-82) in Flow
Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.125 µg of Biotin Rat IgG2a Isotype Control (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD254 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-5952-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
IK22/5
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The IK22/5 monoclonal antibody reacts with mouse CD254 also known as TRANCE (TNF-related activation-induced cytokine receptor) and RANKL (receptor activator of NF-kappa B ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation and is expressed abundantly by mouse T cells not B cells in mouse thymus and lymph nodes. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL.
Applications Reported: This IK22/5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The IK22/5 antibody has been tested by flow cytometric analysis of mouse CD254 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-RANK-L; M-Rank-L; OPG ligand; osteoclast differentiation factor; osteoprotegerin ligand; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TNF-related ligand; tumor necrosis factor-related activation-induced cytokine; unnamed protein product
Gene Aliases: Ly109l; ODF; OPGL; RANKL; Trance
UniProt ID: (Mouse) O35235
Entrez Gene ID: (Mouse) 21943
negative regulation of transcription from RNA polymerase II promoter
ossification
osteoclast proliferation
monocyte chemotaxis
immune response
cell surface receptor signaling pathway
organ morphogenesis
positive regulation of gene expression
cytokine-mediated signaling pathway
calcium-mediated signaling
osteoclast differentiation
tumor necrosis factor-mediated signaling pathway
mammary gland epithelial cell proliferation
positive regulation of homotypic cell-cell adhesion
osteoclast development
paracrine signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
tooth eruption
bone resorption
regulation of osteoclast differentiation
positive regulation of osteoclast differentiation
positive regulation of bone resorption
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
lymph node development
positive regulation of T cell activation
positive regulation of corticotropin-releasing hormone secretion
positive regulation of protein kinase B signaling
calcium ion homeostasis
bone development
mammary gland alveolus development
positive regulation of ERK1 and ERK2 cascade
positive regulation of fever generation by positive regulation of prostaglandin secretion
positive regulation of NIK/NF-kappaB signaling
positive regulation of intracellular signal transduction
cellular response to leukemia inhibitory factor
positive regulation of osteoclast development
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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