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Invitrogen
CD276 (B7-H3) Monoclonal Antibody (M3.2D7), Biotin, eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD276 (B7-H3) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 4
CD276 (B7-H3) Antibody (13-5973-82) in Flow
Staining of non-transfected (left) and mouse B7-H3 transfected (right) 293T cells with 0.25 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD276 (B7-H3) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total cells were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD276 (B7-H3) Antibody (13-5973-82) in Flow")
Product Details
13-5973-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
M3.2D7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The M3.2D7 monoclonal antibody was generated against and reacts with mouse B7-H3, a member of the B7 family of the Ig superfamily proteins. B7-H3 is reported to be expressed by antigen presenting cells and developing bone during embryogenesis. The ligand for B7-H3 has not yet been identified and it is thought that B7-H3 down-regulates T cell functions by engaging an unknown counter receptor on T cells. M3.2D7 stains mouse B7-H3 transfected cells and not mouse spleen cells. M3.2D7 also stains human B7-H3 transfected cells.
Applications Reported: The M3.2D7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The M3.2D7 antibody has been tested by flow cytometric analysis of mB7-H3 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B7 homolog 3; B7-H3; CD276; CD276 antigen; Costimulatory molecule; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276
Gene Aliases: 6030411F23Rik; AU016588; B7h3; B7RP-2; Cd276
UniProt ID: (Mouse) Q8VE98
Entrez Gene ID: (Mouse) 102657
positive regulation of bone mineralization
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
negative regulation of interferon-gamma biosynthetic process
positive regulation of interferon-gamma biosynthetic process
negative regulation of interleukin-2 biosynthetic process
positive regulation of osteoblast differentiation
negative regulation of inflammatory response
regulation of immune response
positive regulation of interleukin-2 secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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