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Invitrogen

IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience™

2 Published Figures
5 References
View all (100) IL-4 antibodies

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Product Details

13-7048-81

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

-
View 2 publications 2 publications

ELISA (ELISA)

0.5-2 µg/mL
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP4-25D2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466904

Product Specific Information

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry.

Applications Tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; IL-4v2; IL4; ILN; Interleukin; interleukin 4; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)
cytokine activity cytokine receptor binding interleukin-4 receptor binding protein binding growth factor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter immune response JAK-STAT cascade cholesterol metabolic process positive regulation of cell proliferation positive regulation of gene expression negative regulation of epithelial cell migration positive regulation of macroautophagy B cell differentiation positive regulation of cell migration positive regulation of B cell proliferation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production positive regulation of interleukin-13 production interleukin-4-mediated signaling pathway type 2 immune response positive regulation of T cell proliferation T cell activation B cell activation macrophage activation myeloid dendritic cell differentiation negative regulation of apoptotic process regulation of isotype switching leukocyte activation positive regulation of MHC class II biosynthetic process positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of receptor-mediated endocytosis positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes negative regulation of inflammatory response regulation of immune response regulation of multicellular organismal process regulation of primary metabolic process dendritic cell differentiation positive regulation of cold-induced thermogenesis neuroinflammatory response negative regulation of neuroinflammatory response positive regulation of beta-amyloid clearance positive regulation of cellular respiration negative regulation of complement-dependent cytotoxicity negative regulation of cellular response to transforming growth factor beta stimulus negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production positive regulation of ATP biosynthetic process
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