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Invitrogen
c-Met Monoclonal Antibody (eBioclone 97), Biotin, eBioscience™
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FIGURE: 1 / 7
c-Met Antibody (13-8858-80) in Flow
Staining of human c-Met-transfected BAF/3 cells with 1.0 µg of Biotin Rat IgG1 Isotype Control (Product # 13-4301-82) (open histogram) or 1.0 µg of Anti-Human c-Met (HGF Receptor) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Product Details
13-8858-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioclone 97
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: 97 monoclonal antibody was generated against a human c-Met-Ig fusion protein, and reacts with human c-Met (HGFR)-transfected cells. Human c-Met is a 145 kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC gamma;. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis. Mutations in human c-Met have been implicated in the development of several malignancies.
Applications Reported: This eBioclone 97 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioclone 97 antibody has been tested by flow cytometric analysis of human c-Met-transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met oncogene; MET proto-oncogene protein; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; tyrosine kinase; hepatocyte growth factor receptor; HGFR; Tyrosine-protein kinase Met; unnamed protein product
Gene Aliases: AUTS9; c-Met; DA11; DFNB97; HGFR; MET; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
ATP binding
kinase activity
transferase activity
semaphorin receptor activity
protein phosphatase binding
identical protein binding
molecular function activator activity
endothelial cell morphogenesis
liver development
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of gene expression
negative regulation of autophagy
neuron differentiation
pancreas development
positive regulation of microtubule polymerization
negative regulation of Rho protein signal transduction
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
cell development
animal organ development
branching morphogenesis of an epithelial tube
positive chemotaxis
negative regulation of stress fiber assembly
excitatory postsynaptic potential
establishment of skin barrier
negative regulation of thrombin receptor signaling pathway
semaphorin-plexin signaling pathway
negative regulation of hydrogen peroxide-mediated programmed cell death
negative regulation of guanyl-nucleotide exchange factor activity
positive regulation of endothelial cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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