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Blood Group Antigen H (O) Type 1 Monoclonal Antibody (17-206), Biotin, eBioscience™
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FIGURE: 1 / 1
Blood Group Antigen H (O) Type 1 Antibody (13-9810-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human kidney medulla using 10 µg/mL of Anti-Human Blood Group Antigen H (O) Type 1 Antigen Biotin followed by DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
13-9810-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
17-206
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 17-206 recognizes the human blood group antigen H (O) type 1, a subtype primarily found in epithelial and secretory cells. The precursor H antigen is modified by addition of saccharides to carbohydrate chains of proteins and lipids on erythrocytes and epithelial cells. The type, attachment, location, and sequence of saccharides determines the blood group antigen subtype and is mediated by multiple glycosyl transferases. Depending on ABO blood type, the H antigen is converted into either the A antigen, B antigen, both the A and B antigen, or left unconverted (O antigen). Evidence that the blood group antigen H is not expressed in tumor cells and that the level correlates with disease progression suggests that the blood group antigen H may be involved in cell proliferation, cell adhesion, and angiogenesis, although its exact function remains to be determined. The 17-206 antibody does not recognize blood group antigen H (O) type 2.
Applications Reported: This 17-206 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This 17-206 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using either high or low pH antigen retrieval at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal perfomance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The H antigen is a precursor to the ABO blood group antigens, present in people of all common blood types. The Bombay phenotype (hh) does not express antigen H on red blood cells, and therefore this type will also lack A or B antigens, similar to the O blood group. However, unlike O group, the H antigen is absent, hence the individuals produce isoantibodies to antigen H as well as to both A and B antigens. If they receive blood from someone with O blood group, the anti-H antibodies will bind to the H antigen on the red blood cells of the donor blood and destroy the RBCs by complement-mediated lysis. Therefore, people with Bombay phenotype can receive blood only from other hh donors, although they can donate as though they were type O. Some individuals with the blood group A1 may also be able to produce anti-H antibodies due to the complete conversion of all the H antigen to A1 antigen. Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19. The H locus contains three exons that span more than 5 kb of genomic DNA, and encodes the fucosyltransferase that produces the H antigen on RBCs. The H antigen is a carbohydrate sequence with carbohydrates linked mainly to protein (with a minor fraction attached to ceramide moiety).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABH; BG-H; BG-H1; group h
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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