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CD15 Monoclonal Antibody (HI98), eBioscience™
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FIGURE: 1 / 7
CD15 Antibody (14-0159-80) in Flow
THP-1 and Ramos cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-000) and then stained with CD15 Monoclonal Antibody (HI98), eBioscience™ (Product # 14-0159-82) at a concentration of 0.25 µg (red histogram) and CD19 Monoclonal Antibody (HIB19), FITC, eBioscience™ (Product # 11-0199-42) (green histogram) at a concentration of 1 µg or left unstained (black histogram), followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 (P... View More
Product Details
14-0159-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgM, kappa
Class
Monoclonal
Type
Antibody
Clone
HI98
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P).
Applications Tested: The HI98 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The HI98 antibody has also been tested by immunohistochemistry on FFPE tisue (IHC-P) at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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